Mouse MIP3 alpha ELISA Kit (CCL20) (ab100728)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.06 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse MIP3 alpha ELISA Kit (CCL20)
Macrophage Inflammatory Protein 3 alpha キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3 pg/ml -
検出範囲
2.06 pg/ml - 1500 pg/ml -
添加回収試験
90 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 80.52 72% - 91% Serum 83.74 74% - 93% Plasma 108.2 84% - 143% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s MIP3 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP3 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 unit 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MIP3 alpha 2 vials MIP3 alpha Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
組織特異性
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Beta chemokine exodus 1
- Beta-chemokine exodus-1
- C C motif chemokine ligand 20
see all -
参照データベース
- Entrez Gene: 20297 Mouse
- SwissProt: O89093 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100728 は 1 報の論文で使用されています。
- Sakamoto K et al. Disruption of the endopeptidase ADAM10-Notch signaling axis leads to skin dysbiosis and innate lymphoid cell-mediated hair follicle destruction. Immunity 54:2321-2337.e10 (2021). PubMed: 34582748