Mouse MCP5 ELISA Kit (ab100723)
Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 0.41 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse MCP5 ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.5 pg/ml -
検出範囲
0.41 pg/ml - 100 pg/ml -
添加回収試験
93 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95.34 84% - 105% Serum 92.42 81% - 103% Plasma 94.14 82% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s MCP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP5 in serum, plasma and cell culture supernates.
This assay employs an antibody specific for mouse MCP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MCP5 2 vials MCP5 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MCP5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
MCP5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations -
別名
- C C motif chemokine 12
- Ccl12
- MCP 1 related chemokine
see all -
参照データベース
- Entrez Gene: 20293 Mouse
- SwissProt: Q62401 Mouse
画像
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Typical standard curve
Representative standard curve using ab100723
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Typical standard curveRepresentative standard curve using ab100723
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100723 は論文での使用が確認できていません。