Mouse MCP1 ELISA Kit (ab208979)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.487 pg/ml
- Range: 1.56 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse MCP1 ELISA Kit
MCP1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Mouse serum 8 4.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Mouse serum 3 9.9% -
サンプルの種類
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.487 pg/ml -
検出範囲
1.56 pg/ml - 100 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 98 91% - 107% Serum 105 101% - 112% Hep Plasma 105 103% - 108% EDTA Plasma 106 104% - 110% Cit plasma 104 100% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse
非交差種: Goat, Cow, Pig -
製品の概要
Mouse MCP1 ELISA Kit (ab208979) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP1 with 0.487 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
MCP1 (CCL2) is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. MCP1 is involved in the recruitment of monocytes and lymphocytes and in the regulation of Th1/Th2 lymphocyte differentiation.
The standard is affinity purified.
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試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Mouse MCP1 Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Mouse MCP1 Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 5BI 1 x 6ml 10 x 6ml 1 x 6ml Mouse MCP1 Lyophilized Recombinant Protein (ab9901) 2 vials 10 vials 2 vials Plate Seals 1 unit 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 1 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究分野
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機能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all -
参照データベース
- Entrez Gene: 20296 Mouse
- SwissProt: P10148 Mouse
- Unigene: 290320 Mouse
関連製品
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Alternative Versions
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Matched antibody pairs
画像
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Standard curve comparison between mouse MCP1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold increase in sensitivity.
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The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and RAW264.7 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 157.7 pg/mL in serum, 125.7 pg/mL in plasma (citrate), 121.1 pg/mL in plasma (EDTA), and 277.2 pg/mL in RAW264.7 cell culture supernatant.
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Background-subtracted data values (mean +/- SD) are graphed.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab208979 は 14 報の論文で使用されています。
- Yu Y et al. MicroRNA-665-3p exacerbates nonalcoholic fatty liver disease in mice. Bioengineered 13:2927-2942 (2022). PubMed: 35038955
- Chen TH et al. Oligosaccharides Ameliorate Acute Kidney Injury by Alleviating Cluster of Differentiation 44-Mediated Immune Responses in Renal Tubular Cells. Nutrients 14:N/A (2022). PubMed: 35215410
- Kim HJ et al. ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation. Cell Mol Life Sci 79:155 (2022). PubMed: 35218410
- Welch GM et al. Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration. Sci Adv 8:eabo4662 (2022). PubMed: 36170369
- Wang YX et al. Mechanism of M2 macrophage-derived extracellular vesicles carrying lncRNA MEG3 in inflammatory responses in ulcerative colitis. Bioengineered 12:12722-12739 (2021). PubMed: 34895044