Mouse liver tissue lysate - total protein (7 days) (ab7266)

Q&A (1)

製品の概要

  • 製品名

    Mouse liver tissue lysate - total protein (7 days)
    Liver ライゼート 製品一覧

  • 製品の詳細

    Mouse liver total protein lysate

  • 特記事項

    Source: Outbred NIH-Swiss Albino mice (7 days old)


    The mouse liver tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Constituents: 60.05% Water, 12.5% Glycerol, 9% Tris HCl, 7.7% DTT, 4.4% Sodium chloride, 1% Triton-X-100, 1% Sodium deoxycholate, 1.1% Sodium lauryl sulfate, 0.15% EDTA disodium salt, 0.5% Aprotinin, 0.5% Leupeptin hemisulfate, 0.09% PMSF, 0.01% Bromophenol blue
  • Concentration information loading...

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Mouse liver tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab7266 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7266 は論文での使用が確認できていません。

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Q&A
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Question

Inquiry: Hi, I have some anti-scavenger BI + BII antibody (ab36970) and I want to prepare a positive control to use with it. On the data sheet total mouse liver lysate was used. Could you tell me how this liver lysate was prepared? Thanks

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Answer

Thank you for your enquiry

We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

I reviewed the literature and found the following protocol that you may find useful:

1. Used 1-2 liver lobes.
2. Homogenize tissue in PBS/Protease Inhibitor cocktail.
3. After centrifugation resuspend the pellet in approx. 400µl RIPA lysis buffer 30min (vortex every 5-10 min).
4. Centrifuge to give whole cell extracts.
5. To improve the amount of sample extracted, repeat this lysis step with the pellet from the centrifugation and pool the supernatant of the 1st and the 2nd lysis.

We also stock a range of liver lysates:

Liver (Mouse) Whole Cell Lysate - normal tissue, 0 days old
www.abcam.com/ab7264

Liver (Mouse) Whole Cell Lysate - normal tissue, 7 days old
www.abcam.com/ab7266

Liver (Mouse) Whole Cell Lysate - normal tissue
www.abcam.com/ab29301

Liver (Human) Tissue Lysate - adult normal tissue
www.abcam.com/ab29889

Please let me know if there is any more information you need.

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