製品の概要

  • 製品名
    Mouse Leptin ELISA Kit
    Leptin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Serum 5 5.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Serum 3 11.5%
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Citrate Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    33.6 pg/ml
  • 検出範囲
    93.75 pg/ml - 6000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Urine 80 77% - 82%
    Serum 111 110% - 114%
    Cell culture media 108 107% - 108%
    Heparin Plasma 106 94% - 116%
    Citrate Plasma 104 100% - 111%
    serum free media 104 103% - 105%

  • 全工程の試験時間
    1h 30m
  • ステップ
    One step assay
  • 種交差性
    交差種: Mouse, Rat
    非交差種: Rabbit, Goat, Guinea pig, Cow, Dog, Pig
  • 製品の概要

    Leptin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin protein in mouse serum, plasma (citrate), urine and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause obesity. Circulating levels of Leptin are regulated by food intake, insulin levels and pregnancy status. Mouse leptin has 96% and 85% protein sequence identity to rat and human leptin, respectively.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate (12 x 8 well strips)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab199082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 4514.3 pg/mL in mouse serum and 2698.1 pg/mL in mouse plasma (Citrate).

  • The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean Leptin concentration was determined to be 2983 pg/mL in rat serum, 1626 pg/mL in rat plasma (citrate) and 2134 pg/mL in rat plasma (heparin).

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参考文献

This product has been referenced in:
  • Matsui S  et al. Neuronal SIRT1 regulates macronutrient-based diet selection through FGF21 and oxytocin signalling in mice. Nat Commun 9:4604 (2018). Read more (PubMed: 30389922) »
See 1 Publication for this product

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