Mouse Leptin ELISA Kit (ab108881)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.375 ng/ml - 24 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Leptin ELISA Kit
Leptin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.1% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.3 ng/ml -
検出範囲
0.375 ng/ml - 24 ng/ml -
全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Leptin mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of leptin levels in plasma, serum and cell culture supernatants.
A Leptin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Leptin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leptin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Mouse Leptin Antibody 1 x 90µl Chromogen Substrate 1 x 7ml Leptin Microplate (12 x 8 well strips) 1 unit Leptin Standard 1 x 2 vials Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
関連疾患
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the leptin family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- FLJ94114
- LEP
- LEP_HUMAN
see all -
参照データベース
- Entrez Gene: 16846 Mouse
- SwissProt: P41160 Mouse
- SwissProt: P41160 Mouse
- Unigene: 277072 Mouse
画像
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Typical Standard Curve
Representative Standard Curve using ab108881
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108881 は 1 報の論文で使用されています。
- Cui XB et al. RGC32 deficiency protects against high-fat diet-induced obesity and insulin resistance in mice. J Endocrinol 224:127-37 (2015). Functional Studies ; Mouse . PubMed: 25385871