製品の概要

  • 製品名

    Mouse Interferon gamma ELISA Kit (IFNG)
    Interferon gamma キット 製品一覧
  • 検出方法

    Colorimetric
  • サンプルの種類

    Cell culture extracts, Tissue Extracts
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 10 pg/ml
  • 検出範囲

    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    92 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 91.95 84% - 104%
    Tissue Extracts 93.47 83% - 103%

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    Abcam’s Interferon gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Interferon gamma in cell lysate and tissue lysate.

    This assay employs an antibody specific for Mouse Interferon gamma coated on a 96- well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2X Cell Lysis Buffer 1 x 5ml
    440X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-Mouse Interferon gamma (lyophilized) 2 vials
    Interferon gamma Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse Interferon gamma Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 組織特異性

    Released primarily from activated T lymphocytes.
  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 配列類似性

    Belongs to the type II (or gamma) interferon family.
  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve

プロトコール

参考文献

This product has been referenced in:

  • You P  et al. Local level of TGF-ß1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis. Exp Ther Med 15:3639-3649 (2018). Read more (PubMed: 29545894) »
  • Zhou J  et al. Prognostic and therapeutic value of CD103+ cells in renal cell carcinoma. Exp Ther Med 15:4979-4986 (2018). Read more (PubMed: 29805521) »
See all 3 Publications for this product

レビューと Q&A

Answer

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas específicos para ratón, y específicos para humano. Después de estudiar la homología entre secuencias, me temo que no podemos predecir que exista reactividad cruzada entre especies. Experimentalmente tampoco tenemos datos para confirmarlo.

Siento no poder ser de mas ayuda. No dudes en contactarnos para cualquier otra consulta.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録