Mouse Interferon gamma ELISA Kit (IFNG) (ab100689)

Reviews (2)Q&A (3)References (3)

製品の概要

  • 製品名

    Mouse Interferon gamma ELISA Kit (IFNG)
    Interferon gamma キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Cell culture supernatant, Serum, Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    < 5 pg/ml

  • 検出範囲

    2.74 pg/ml - 2000 pg/ml

  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.75 83% - 103%
    Serum 95.38 83% - 103%
    Plasma 93.49 82% - 102%

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Mouse

  • 製品の概要

    Abcam’s Interferon Gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Interferon Gamma in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Interferon Gamma coated on a 96- well plate. Standards and samples are pipetted into the wells and Interferon Gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Interferon Gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon Gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    440X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Interferon gamma (lyophilized) 2 vials
    Interferon gamma Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse Interferon gamma Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • 組織特異性

    Released primarily from activated T lymphocytes.

  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • 配列類似性

    Belongs to the type II (or gamma) interferon family.

  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all

  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab100689 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100689

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100689

    プロトコール

    参考文献

    This product has been referenced in:

    • Chang RC  et al. Preconception paternal alcohol exposure exerts sex-specific effects on offspring growth and long-term metabolic programming. Epigenetics Chromatin 12:9 (2019). Read more (PubMed: 30670059) »
    • Pan Y  et al. Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities. Nutrients 10:N/A (2018). Read more (PubMed: 30201943) »
    See all 3 Publications for this product

    ab100689 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    IFN-gamma ELISA kit

     Average Good 4/5 (Ease of Use)
    Abreviews
    abreview image
    The kit is ok but with some of non-specific bindings. Still got satisfactory results.

    Miss. Sheelu Monga

    Verified customer

    投稿 May 08 2018

    GOOD

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Sample: Mouse Cell (Homogenised lung tissue)
    Specification: Homogenised lung tissue
    Type of Kit Used: ELISA
    Positive control: Virally infected lung tissue.
    Did you use the Abcam Kit protocol?: Yes
    Additional data
    Additional Notes: Overall, a good kit.

    Abcam user community

    Verified customer

    投稿 Jan 27 2016

    Question

    Hola,
    Estoy buscando kits de ELISA para detección de citocinas (en particular, IL-1 beta, IL-17 e IFN gamma, pero si van bien, luego podrían ser más), pero necesitaría que detectaran citocinas tanto humanas como de ratón. Veo que ustedes tienen varios kits, pero todos reconocen la proteína humana o la de ratón. Ustedes tienen información sobre reacción cruzada? Es posible que alguno de estos kits reconozca las dos proteínas?
    Gracias!

    Read More
    Answer

    Gracias por contactarnos.


    He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

    Tenemos varios kits contra dichas citoquinas específicos para ratón, y específicos para humano. Después de estudiar la homología entre secuencias, me temo que no podemos predecir que exista reactividad cruzada entre especies. Experimentalmente tampoco tenemos datos para confirmarlo.

    Siento no poder ser de mas ayuda. No dudes en contactarnos para cualquier otra consulta.

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    Question

    looking for ELISA kit for IL2, IL4, IL10 and IFN that works both with human and mouse
    are any of these kits crossreactive with human or mouse?

    Read More
    Answer

    I have broken down the cross-reactivity information for each kit below:


    · ab100549 IL10 Human ELISA Kit
    o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected to cross react with mouse samples.
    · ab100566 IL2 Human ELISA Kit
    o The capture antibody did not show any cross-reactivity with Murine IL-2 from testing. Thus, this kit is not expected to cross react with mouse samples.
    · ab100570 IL4 Human ELISA Kit
    o Capture Antibody
    § Mouse, monoclonal
    § Not tested for cross-reactivity with mouse samples.
    o Detection Antibody
    § Goat, polyclonal
    § Detects the region His25­Ser153 (Accession # NP_000580)
    § Not tested for cross-reactivity with mouse samples.
    · ab100689 Interferon gamma Mouse ELISA Kit
    o Capture Antibody
    § Host species: Rat
    § Not tested for cross-reactivity with human samples.
    o Detection Antibody
    § Host species: Rat
    § Not tested for cross-reactivity with human samples.
    · ab100697 IL10 Mouse ELISA Kit
    o Capture Antibody
    § Rat, monoclonal
    § Not tested for cross-reactivity with human samples.
    o Detection Antibody
    § Rat, monoclonal
    § Not tested for cross-reactivity with human samples.
    · ab100706 IL2 Mouse ELISA Kit
    o The detection antibody did not show any cross-reactivity with Human IL-2 from testing. Thus, this kit is not expected to cross react with human samples.
    · ab100710 IL4 Mouse ELISA Kit
    o The capture antibody did not show any cross-reactivity with Human IL-4 from testing. Thus, this kit is not expected to cross react with human samples.

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    Question

    Our customer is looking for  IFN gamma ELISA kit for bovine and guinea pig.   His samples are serum and plasma.   I have checked there's no product for bovine and guinea pig sample but,   please check the product which has possibility to cross react.

    Read More
    Answer

    Thank you for contacting us.   We have the following products in our catalog: ab108863: Interferon gamma Human ELISA Kit We have tested the cross-reactivity with bovine samples and it is very low, around 1 %. We have never tested samples from guinea pig. ab100537: Interferon gamma Human ELISA Kit - 1 x 96 Well Plate ab100689: Interferon gamma Mouse ELISA Kit - 1 x 96 Well Plate Guinea pig IFN-gamma shares ~56% and ~41% to human and mouse respectively. Therefore this protein doesn’t seem to be that conserved between species and as these ELISA kits are sandwich-based and very species specific, I would expect minimal to zero cross reactivity with a guinea pig sample. The only way to know for sure would be to test and see. However, we might be able to add a bovine IFN-gamma ELISA Kit to our catalog. I will check and let you know. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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