Mouse Insulin like Growth Factor 1 ELISA kit (IGF1) (ab108874)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.3 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Insulin like Growth Factor 1 ELISA kit (IGF1)
IGF1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 3.9% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 8.6% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.12 ng/ml -
検出範囲
0.3 ng/ml - 5 ng/ml -
添加回収試験
96 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Insulin like Growth Factor 1 (IGF1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 levels in plasma, serum and cell culture supernatants.
An IGF1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IGF1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IGF1 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Mouse Insulin like Growth Factor 1 Antibody 1 x 150µl Chromogen Substrate 1 x 7ml Insulin like Growth Factor 1 Microplate (12 x 8 well strips) 1 unit Insulin like Growth Factor 1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
関連疾患
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
配列類似性
Belongs to the insulin family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- IBP1
- IGF I
- IGF IA
see all -
参照データベース
- Entrez Gene: 16000 Mouse
- SwissProt: P05017 Mouse
- Unigene: 268521 Mouse
画像
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Typical Standard Curve
Representative Standard Curve using ab108874
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108874 は 2 報の論文で使用されています。
- Wu H et al. Whole Body Vibration Retards Progression of Atherosclerosis via Insulin-Like Growth Factor 1 in Apolipoprotein E-Deficient Mice. Biomed Res Int 2018:4934861 (2018). PubMed: 29707570
- Wang T et al. GDF15 is a heart-derived hormone that regulates body growth. EMBO Mol Med 9:1150-1164 (2017). PubMed: 28572090