製品の概要

  • 製品名

    Mouse IL-9 ELISA Kit
    IL-9 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 2.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 4.3%
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Hep Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    1.67 pg/ml
  • 検出範囲

    3.1 pg/ml - 200 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 97 92% - 101%
    Urine 80 76% - 83%
    Serum 116 110% - 123%
    Hep Plasma 110 98% - 122%
    Cit plasma 110 100% - 127%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse
    非交差種: Cow
  • 製品の概要

    IL-9 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-9 protein in mouse, plasma, urine, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-9 is a cytokine produced by CD4+ helper T cells, and it regulates hematopoietic cells through cell proliferation and the prevention of apoptosis.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Mouse IL-9 Capture Antibody 1 x 600µl
    10X Mouse IL-9 Detector Antibody 1 x 600µl
    Mouse IL-9 Lyophilized Recombinant Protein 2 vials
    Antibody Diluent CPR 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • 研究分野

  • 機能

    Supports IL-2 independent and IL-4 independent growth of helper T-cells.
  • 配列類似性

    Belongs to the IL-7/IL-9 family.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Cytokine P40
    • Homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL 9
    • IL-9
    • Il9
    • IL9_HUMAN
    • Interleukin 9
    • Interleukin-9
    • Mast cell growth factor
    • MCGF
    • Megakaryoblast growth factor
    • P40
    • p40 cytokine
    • p40 T cell and mast cell growth factor
    • T cell growth factor 3
    • T cell growth factor p40
    • T-cell growth factor P40
    • TCGF 3
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab222505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, urine 25%, and EL4 supernatant 50%.

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参考文献

ab222505 has not yet been referenced specifically in any publications.

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