製品の概要

  • 製品名

    Mouse IL-6 ELISA Kit
    IL-6 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Spleen CCS 5 3.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Spleen CCS 3 3.4%
  • サンプルの種類

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    11.3 pg/ml
  • 検出範囲

    15.6 pg/ml - 1000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 107 103% - 114%
    Cell culture media 100 89% - 112%
    Heparin Plasma 98 95% - 103%
    EDTA Plasma 112 109% - 116%
    Citrate Plasma 99 96% - 102%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human
  • 製品の概要

    IL-6 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 protein in serum, plasma and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Mouse IL-6 Capture Antibody 1 x 600µl
    10X Mouse IL-6 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse IL-6 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 関連疾患

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 配列類似性

    Belongs to the IL-6 superfamily.
  • 翻訳後修飾

    N- and O-glycosylated.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab222503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 39 pg/mL in serum, 42 pg/mL in plasma (heparin), 43 pg/mL in plasma (EDTA), and 32 pg/mL in plasma (citrate).

  • The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated RAW264.7 cell culture supernatant 1:50 and stimulated spleen cell culture supernatant 1:16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 26,793 pg/mL in stimulated RAW264.7 cell culture supernatant, and 15,127 pg/mL in stimulated spleen cell culture supernatant. 

プロトコール

参考文献

This product has been referenced in:

  • Ren LR  et al. Staphylococcus Aureus Induces Osteoclastogenesis via the NF-?B Signaling Pathway. Med Sci Monit 23:4579-4590 (2017). Read more (PubMed: 28942456) »
  • Luo D  et al. Natural product celastrol suppressed macrophage M1 polarization against inflammation in diet-induced obese mice via regulating Nrf2/HO-1, MAP kinase and NF-?B pathways. Aging (Albany NY) 9:2069-2082 (2017). Read more (PubMed: 29040966) »
See all 3 Publications for this product

レビューと Q&A

IL-6 ELISA kit simple step

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Very good kit and easy to use. I would highly recommend this kit.

Miss. Sheelu Monga

Verified customer

投稿 Apr 27 2018

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