Mouse IL-6 ELISA Kit (ab100713)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 600 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-6 ELISA Kit
IL-6 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
0.82 pg/ml - 600 pg/ml -
添加回収試験
93 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture extracts 94.53 84% - 104% Tissue Extracts 93.39 83% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-6 in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Mouse IL-6 2 vials IL-6 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-6 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
画像
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IL-6 measured in mouse tissue lysates prepared with RIPA buffer and sonicated in an ice-cold water bath for 12 intervals of a total of 3.5 minutes. 0.02-1 mg protein x mL-1 were tested, results shown as IL-6 per mg of extracted protein (duplicates +/- SD).
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IL-6 measured in RIPA lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. Samples were diluted and tested in the range of 1e4-5e5 cells x mL-1, results expressed per million cells; duplicates +/- SD).
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Dilution curves of mouse IL-6 (ab100713) and NIBSC standard (93/730). One pg of standard mouse IL-6 corresponds to 0.37 (+/- 0.07) AU. Background signal subtracted (duplicates; +/- SD).
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Standard curve of mouse IL-6 with background signal subtracted (duplicates; +/- SD).
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Representative standard curve using ab100713
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (66)
ab100713 は 66 報の論文で使用されています。
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- Alsabaani NA et al. Maslinic Acid Protects against Streptozotocin-Induced Diabetic Retinopathy by Activating Nrf2 and Suppressing NF-κB. J Ophthalmol 2022:3044202 (2022). PubMed: 35265366
- Tang W et al. METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathy. BMC Nephrol 23:124 (2022). PubMed: 35354439
- Ivshina MP et al. CPEB1 regulates the inflammatory immune response, phagocytosis, and alternative polyadenylation in microglia. Glia 70:1850-1863 (2022). PubMed: 35635122
- Wang C et al. Effects of Tea Treatments against High-Fat Diet-Induced Disorder by Regulating Lipid Metabolism and the Gut Microbiota. Comput Math Methods Med 2022:9336080 (2022). PubMed: 35677179