Mouse IL-6 ELISA Kit (ab100712)
製品の概要
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製品名
Mouse IL-6 ELISA Kit
IL-6 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
0.82 pg/ml - 600 pg/ml -
添加回収試験
94 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.18 86% - 103% Serum 93.39 83% - 103% Plasma 94.53 84% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse
交差が予測される動物種: Human
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製品の概要
Mouse IL-6 ELISA kit is designed for the quantitative measurement of Mouse IL-6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-mouse IL-6 (Lyophilized) 2 vials IL-6 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-6 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- Entrez Gene: 3569 Human
- Entrez Gene: 16193 Mouse
- Omim: 147620 Human
- SwissProt: P05231 Human
- SwissProt: P08505 Mouse
- Unigene: 654458 Human
- Unigene: 1019 Mouse
画像
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Sandwich ELISA - IL-6 (Interleukin-6) Mouse ELISA Kit (ab100712)Standard curve of msIL-6 in different diluents with background signal subtracted (duplicates; +/- SD). -
Sandwich ELISA - IL-6 (Interleukin-6) Mouse ELISA Kit (ab100712)IL-6 measured in mouse tissue lysates (diluted in the range of 1:20 to 1:200; expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
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Sandwich ELISA - IL-6 (Interleukin-6) Mouse ELISA Kit (ab100712)msIL-6 measured in biological fluids, background signal subtracted (duplicates +/- SD). -
Sandwich ELISA - IL-6 (Interleukin-6) Mouse ELISA Kit (ab100712)IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD). -
Typical standard curveRepresentative standard curve using ab100712
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Typical standard curveRepresentative standard curve using ab100712
参考文献
ab100712 は 51 報の論文で使用されています。
- Gao J et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
- Liu T et al. Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1. J Cell Mol Med 24:2189-2201 (2020). PubMed: 31957232
- Ma Y et al. Mangiferin Relieves Lipopolysaccharide-Induced Injury by Up-Regulating miR-181a via Targeting PTEN in ATDC5 Cells. Front Pharmacol 11:137 (2020). PubMed: 32210798
- Zhao Y et al. Surface-anchored framework for generating RhD-epitope stealth red blood cells. Sci Adv 6:eaaw9679 (2020). PubMed: 32219154
- Cabrera-Aguilera I et al. Chronic Sleep Fragmentation Mimicking Sleep Apnea Does Not Worsen Left-Ventricular Function in Healthy and Heart Failure Mice. Front Neurol 10:1364 (2019). PubMed: 31993015
