製品の概要

  • 製品名
    Mouse IL-4 ELISA Kit
    IL-4 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Supernatant 3 8.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Supernatant 5 6.6%
  • サンプルの種類
    Serum, Cell culture extracts, Cell culture media
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    6.3 pg/ml
  • 検出範囲
    18.75 pg/ml - 1200 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 84 75% - 95%
    Cell culture extracts 111 103% - 118%
    Cell culture media 105 94% - 114%

  • 全工程の試験時間
    1h 30m
  • ステップ
    One step assay
  • 種交差性
    交差種: Mouse
  • 製品の概要

    IL-4 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 protein in mouse serum, cell culture supernatant, and cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 6.3 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR - 13.7 pg/mL
    Samples diluted in Sample Diluent NBS - 6.8 pg/mL

  • 特記事項

    Mouse IL-4 is a 120 amino acids long secreted protein. IL-4 is a cytokine produced mostly by activated T lymphocytes, mast cells and basophils. It has variety of immune response modulating functions by acting on number of cell types. IL-4 participates in several B-cell activation processes. It is a co-stimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

  • アプリケーション
    適用あり: ELISAmore details
  • 試験プラットフォーム
    Microplate (12 x 8 well strips)

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Mouse IL-4 Capture Antibody 1 x 600µl
    10X Mouse IL-4 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Mouse IL-4 Lyophilized Recombinant Protein 1 x 2 vials
    Plate Seals 1 unit
    Sample Diluent NBS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野
  • 機能
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • 関連疾患
    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • 配列類似性
    Belongs to the IL-4/IL-13 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • B cell growth factor 1
    • B cell IgG differentiation factor
    • B Cell Stimulatory Factor 1
    • B-cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF-1
    • BSF1
    • IGG1 induction factor
    • IL 4
    • IL-4
    • IL4
    • IL4_HUMAN
    • Il4e12
    • Interleukin 4
    • Interleukin 4 variant 2
    • Interleukin 4, isoform 1
    • Interleukin-4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab221833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

画像

  • Comparison of IL-4 in unstimulated and PHA+PMA stimulated EL4.IL2 cell supernatants. EL4.IL2 cells were cultured for 2 days in the presence or absence of 10 µg/mL PHA and 10 ng/mL PMA. The concentrations of IL-4 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-4 concentration was determined to be 3549 pg/mL in PHA+PMA stimulated EL4.IL2 cell culture supernatant and undetectable in unstimulated EL4.IL2 cell culture supernatant and DMEM media (not shown).

  • Interpolated concentrations of native IL-4 in PHA+PMA treated EL4.IL2 cell extract samples based on a 500 μg/mL extract load. The concentrations of (target) were measured in duplicate and interpolated from the IL-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 822.0 pg/mL in PHA+PMA treated cell extract samples and undetectable in untreated EL4.IL2 cell extract samples (results not shown).

  • Interpolated concentrations of native IL-4 in PHA+PMA treated EL4.IL2 cell culture supernatant (4 days) samples. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: EL4.IL2 cell supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 3529 pg/mL in neat PHA+PMA treated EL4.IL2 cell culture supernatant.

  • Interpolated concentrations of spike IL-4 in mouse serum samples. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Example of mouse IL-4 standard curve in Sample Diluent NBS.

  • Example of mouse IL-4 standard curve in Sample Diluent NS.

プロトコール

参考文献

ab221833 has not yet been referenced specifically in any publications.

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