Mouse IL-21 ELISA Kit (ab100707)

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製品の概要

  • 製品名

    Mouse IL-21 ELISA Kit
    IL-21 キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Cell culture supernatant, Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    < 6 pg/ml

  • 検出範囲

    8.19 pg/ml - 2000 pg/ml

  • 添加回収試験

    89 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.65 87% - 109%
    Plasma 77.38 68% - 87%

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Mouse

  • 製品の概要

    Abcam’s IL-21 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in plasma and cell culture supernatants. (Mouse IL-21 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Mouse IL-21 in normal serum with ab100707, only in serum spiked with Mouse IL-21.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse IL-21 2 vials
    IL-21 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-21 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • 研究分野

  • 関連性

    A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. IL-21 has been found to be a powerful growth factor for naive B cells. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAKSTAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

  • 細胞内局在

    Secreted

  • 別名

    • Cytokine CX2
    • DN 452A22.7
    • IL 21
    • IL21
    • Interleukin 21
    • Interleukin 21 isoform
    • Interleukin21
    • OTTMUSP00000007838
    • Za11
    see all

  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab100707 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100707

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100707

    プロトコール

    参考文献

    ab100707 has not yet been referenced specifically in any publications.

    ab100707 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    starting dilution for plasma samples?

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    Answer


    We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

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    Question

    Product code: 100707
    Inquiry: Hi, I am interested in your mouse IL-21 ELISA kit. I would like to konw if the antibodies provided with the kit are polyclonal or monoclonal. Hope you can help, and thanks.

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    Answer

    Thank you for your enquiry.

    I can confirm that both the antibodies provided with ab100707 IL21 Mouse ELISA Kit are polyclonal.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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