Mouse IL-2 ELISA Kit (ab100706)

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製品の概要

  • 製品名

    Mouse IL-2 ELISA Kit
    IL-2 キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Cell culture supernatant, Serum, Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    < 1 pg/ml

  • 検出範囲

    0.625 pg/ml - 40 pg/ml

  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 100.34 89% - 110%
    Serum 97.56 85% - 104%
    Plasma 98.34 86% - 106%

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Mouse

  • 製品の概要

    Abcam’s IL-2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100706

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100706

プロトコール

参考文献

This product has been referenced in:

  • Alam MF  et al. Therapeutic potential of Vanillylacetone against CCl4 induced hepatotoxicity by suppressing the serum marker, oxidative stress, inflammatory cytokines and apoptosis in Swiss albino mice. Exp Mol Pathol 105:81-88 (2018). Read more (PubMed: 29909158) »
See 1 Publication for this product

ab100706 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

looking for ELISA kit for IL2, IL4, IL10 and IFN that works both with human and mouse
are any of these kits crossreactive with human or mouse?

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Answer

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100566 IL2 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-2 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100570 IL4 Human ELISA Kit
o Capture Antibody
§ Mouse, monoclonal
§ Not tested for cross-reactivity with mouse samples.
o Detection Antibody
§ Goat, polyclonal
§ Detects the region His25­Ser153 (Accession # NP_000580)
§ Not tested for cross-reactivity with mouse samples.
· ab100689 Interferon gamma Mouse ELISA Kit
o Capture Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
· ab100697 IL10 Mouse ELISA Kit
o Capture Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
· ab100706 IL2 Mouse ELISA Kit
o The detection antibody did not show any cross-reactivity with Human IL-2 from testing. Thus, this kit is not expected to cross react with human samples.
· ab100710 IL4 Mouse ELISA Kit
o The capture antibody did not show any cross-reactivity with Human IL-4 from testing. Thus, this kit is not expected to cross react with human samples.

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Question

Hi,
thanks a lot for the fast reply, I want toknowifI can use the abcam kitab100710, ab100706 and ab100697on serum that been storage at -20C and -80C for 2 months

thank you

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Answer

Thank you for your inquiry.

I can confirm that these kit are suitable for the use with serum samples that have been frozen.

I recommend to not to mix/compare samples thathave been frozen with samples that have not been frozen unless the impact of freezing is tested in a control experiment first.

I hope this information is helpful and wish you good luck with your experiments.

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