Mouse IL-18BP ELISA Kit (ab254509)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 151.82 pg/ml
- Range: 468.75 pg/ml - 30000 pg/ml
- Sample type: Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-18BP ELISA Kit
IL-18BP キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 3.7% -
サンプルの種類
Serum, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
151.82 pg/ml -
検出範囲
468.75 pg/ml - 30000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 97 % - % Cit plasma 98 % - % -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse IL-18BP ELISA Kit (ab254509) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cit plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-18BP with 151.82 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
IL-18BP is a protein that binds to IL-18, preventing it from interacting with its receptor. IL-18BP inhibits the early TH1 cytokine response. IL-18 is produced constitutively by mononuclear cells.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 10X Mouse IL-18BP Capture Antibody 1 x 600µl 1 x 6000µl 10X Mouse IL-18BP Detector Antibody 2 vials 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 4BR 1 x 6ml 10 x 6ml Mouse IL-18BP Lyophilized Recombinant Protein 2 vials 2 x 10 vials Plate Seals 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究分野
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機能
Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. -
組織特異性
Strongly expressed in heart, lung, placenta and spleen. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- I18BP_HUMAN
- IL-18BP
- IL18 binding protein
see all -
参照データベース
- Entrez Gene: 16068 Mouse
- SwissProt: Q9Z0M9 Mouse
- Unigene: 422756 Mouse
関連製品
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Alternative Versions
画像
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The IL-18BP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18BP concentration was determined to 175.87 ng/mL in serum and 201.01 ng/mL in plasma (citrate).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab254509 は論文での使用が確認できていません。