製品の概要

  • 製品名

    Mouse IL-17F ELISA Kit
    IL-17F キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 8.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 4.5%
  • サンプルの種類

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    0.1 pg/ml
  • 検出範囲

    9.375 pg/ml - 600 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 118.6 114.8% - 121.8%
    Cell culture media 91.9 84.4% - 98.8%
    Hep Plasma 118.1 107.7% - 124.2%
    EDTA Plasma 116.6 112.8% - 123%
    Cit plasma 103.6 96.8% - 113.2%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse
    非交差種: Goat, Pig
  • 製品の概要

    Abcam’s IL-17F (Interleukin-17F) mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17F protein in mouse serum, plasma and cell culture supernatant samples.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

     

    Sensitivity:

    Samples diluted Sample Diluent NS: 0.1 pg/mL

    Samples diluted Sample Diluent 25BP: 0.4 pg/mL

  • 特記事項

    IL-17F is an IL-17 family member cytokine that stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. IL-17F inhibits angiogenesis. IL-17F is a glycosylated, disulfide-linked homodimer. IL-17F is also present as IL-17A/F heterodimer. IL-17F is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. It is secreted.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab204522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curve and corrected for sample dilution. Note that 1X Diluted samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of IL 17F per mL of neat sample (mean +/- SD, n=2).

  • Mouse spleen cells were cultured in the absence or presence of 10 ng/mL PMA + 10 ng/mL Mouse recombinant IL-1a for 4 days. The cell culture supernatant samples were collected. The concentrations of IL-17F were measured in the diluted supernatants in duplicates, interpolated from the IL-17F standard curve and corrected for sample dilution. Note that 1X Diluted supernatant samples were neat. The interpolated, dilution factor-corrected values are plotted in pg of IL-17F per mL of neat sample (mean +/- SD, n=2).

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参考文献

ab204522 has not yet been referenced specifically in any publications.

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