製品の概要

  • 製品名

    Mouse IL-13 ELISA Kit
    IL-13 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    supernatant 5 2.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    supernatant 3 1.4%
  • サンプルの種類

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    1.2 pg/ml
  • 検出範囲

    1.95 pg/ml - 125 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 108 106% - 111%
    Serum 97 95% - 98%
    Hep Plasma 104 101% - 108%
    EDTA Plasma 98 97% - 99%
    Cit plasma 97 95% - 100%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    IL-13 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 protein mouse in serum, plasma, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate (12 x 8 well strips)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab219634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).

  • The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.

プロトコール

参考文献

ab219634 has not yet been referenced specifically in any publications.

レビューと Q&A

Filter by Ratings

IL-13 ELISA kit

Good Excellent 5/5 (Ease of Use)
Abreviews
Good one. Easy to use and consume less time.

Miss. Sheelu Monga

Verified customer

投稿 Jun 22 2018

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