Mouse IL-13 ELISA Kit (ab100700)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-13 ELISA Kit
IL-13 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
8.23 pg/ml - 2000 pg/ml -
添加回収試験
94 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95.37 84% - 104% Serum 94.42 83% - 103% Plasma 93.56 82% - 102% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-13 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-13 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IL-13 2 vials IL-13 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-13 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
関連疾患
Allergic rhinitis -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Allergic rhinitis
- ALRH
- BHR 1
see all -
参照データベース
- Entrez Gene: 16163 Mouse
- SwissProt: P20109 Mouse
- Unigene: 1284 Mouse
画像
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Typical standard curve
Representative standard curve using ab100700
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Typical standard curveRepresentative standard curve using ab100700
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100700 は 1 報の論文で使用されています。
- Bacsi A et al. Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation. DNA Repair (Amst) 12:18-26 (2013). PubMed: 23127499