Mouse IL-12 p70 ELISA Kit (ab119531)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-12 p70 ELISA Kit
IL-12 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 7 8.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 7 11% -
サンプルの種類
Cell culture supernatant, Serum -
アッセイタイプ
Sandwich (quantitative) -
検出感度
10 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
添加回収試験
106 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 106 95% - 118% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
IL-20 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20 p70 in serum and cell culture supernatants.
IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.
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特記事項
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-mouse IL-12 p70 monoclonal antibody 1 x 70µl Microplate coated with anti-mouse IL-12 p70 monoclonal antibody (12 x 8 wells) 1 unit Mouse IL-12 p70 Standard, lyophilized (2 ng/mL on reconstitution). 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究分野
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機能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CLMF
- CLMF p35
- CLMF p40
see all -
参照データベース
- Entrez Gene: 16159 Mouse
- Entrez Gene: 16160 Mouse
- SwissProt: P43431 Mouse
- SwissProt: P43432 Mouse
- Unigene: 103783 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab119531 は 5 報の論文で使用されています。
- Lo YT et al. Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development. Sci Rep 13:296 (2023). PubMed: 36609424
- Chen HQ et al. Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy. Immun Inflamm Dis 10:e624 (2022). PubMed: 35634952
- Xiu W et al. Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections. Nat Commun 13:3875 (2022). PubMed: 35790729
- Nath A et al. Gold-manganese oxide nanocomposite suppresses hypoxia and augments pro-inflammatory cytokines in tumor associated macrophages. Int Immunopharmacol 57:157-164 (2018). PubMed: 29499453
- Pan Y et al. Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities. Nutrients 10:N/A (2018). PubMed: 30201943