Mouse IL-11 ELISA Kit (ab100698)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.85 ng/ml - 450 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-11 ELISA Kit
IL-11 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 1 ng/ml -
検出範囲
1.85 ng/ml - 450 ng/ml -
添加回収試験
99 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 77.35 70% - 87% Plasma 117.3 95% - 113% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-11 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-11 in plasma and cell culture supernatants. (Mouse IL-11 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Mouse IL-11 in normal serum with ab100698, only in serum spiked with Mouse IL-11.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-mouse IL-11 2 vials IL-11 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-11 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Adipogenesis inhibitory factor
- AGIF
- IL 11
see all -
参照データベース
- Entrez Gene: 16156 Mouse
- SwissProt: P47873 Mouse
- Unigene: 35814 Mouse
画像
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Typical standard curve
Representative standard curve using ab100698.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100698 は 1 報の論文で使用されています。
- Buzzelli JN et al. Overexpression of IL-11 promotes premalignant gastric epithelial hyperplasia in isolation from germline gp130-JAK-STAT driver mutations. Am J Physiol Gastrointest Liver Physiol 316:G251-G262 (2019). PubMed: 30520693