製品の概要

  • 製品名

    Mouse IL-10 ELISA Kit
    IL-10 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 556.8pg/ml 11.6 2%
    B 8 137.4pg/ml 6.8 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 8 548.2pg/ml 12.1 2.2%
    B 8 133.6pg/ml 5.4 4%
  • サンプルの種類

    Cell culture supernatant, Serum
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 20 pg/ml
  • 検出範囲

    31.25 pg/ml - 1000 pg/ml
  • 全工程の試験時間

    3h 45m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    Abcam’s IL-10 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

    This kit will recognize both endogenous and recombinant mouse IL-10.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46103

プロトコール

参考文献

This product has been referenced in:

  • Hernández-Espinosa DR  et al. Role of NADPH oxidase-2 in the progression of the inflammatory response secondary to striatum excitotoxic damage. J Neuroinflammation 16:91 (2019). Read more (PubMed: 30995916) »
  • Liu B  et al. Root Extract of Polygonum cuspidatum Siebold & Zucc. Ameliorates DSS-Induced Ulcerative Colitis by Affecting NF-kappaB Signaling Pathway in a Mouse Model via Synergistic Effects of Polydatin, Resveratrol, and Emodin. Front Pharmacol 9:347 (2018). Read more (PubMed: 29695964) »
See all 5 Publications for this product

レビューと Q&A

Question
Answer

Thank you for your enquiry. The antibodies used in the Murine IL10 ELISA kit are a goat polyclonal as capture antibody, and a rat monoclonal [B-C40] for detection. I hope this was the information you were looking for.

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