Mouse IL-10 ELISA kit (ab108870)
製品の概要
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製品名
Mouse IL-10 ELISA kit
IL-10 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.1% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Tissue -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.03 ng/ml -
検出範囲
0.031 ng/ml - 2 ng/ml -
添加回収試験
97 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-10 Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.
An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse IL-10 Antibody 1 x 120µl Chromogen Substrate 1 x 8ml IL-10 Microplate (12 x 8 well strips) 1 unit IL-10 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究分野
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
参照データベース
- Entrez Gene: 16153 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
アプリケーション
Our Abpromise guarantee covers the use of ab108870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Typical Standard CurveRepresentative Standard Curve using ab108870
プロトコール
参考文献
This product has been referenced in:
- Qian X et al. Hypouricemic and nephroprotective roles of anthocyanins in hyperuricemic mice. Food Funct 10:867-878 (2019). Read more (PubMed: 30693917) »
- Ye YS et al. Antipruritic effects of electroacupuncture on morphine-induced pruritus model mice through the TLR2/4-MyD88-NF-?B pathway. Neuroreport 30:331-337 (2019). Read more (PubMed: 30822282) »
