製品の概要

  • 製品名

    Mouse IL-10 ELISA kit
    IL-10 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 10.1%
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma, Tissue
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    0.03 ng/ml
  • 検出範囲

    0.031 ng/ml - 2 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間

    5h 00m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    Abcam’s IL-10 Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.


    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab108870

プロトコール

参考文献

This product has been referenced in:

  • Qian X  et al. Hypouricemic and nephroprotective roles of anthocyanins in hyperuricemic mice. Food Funct 10:867-878 (2019). Read more (PubMed: 30693917) »
  • Ye YS  et al. Antipruritic effects of electroacupuncture on morphine-induced pruritus model mice through the TLR2/4-MyD88-NF-?B pathway. Neuroreport 30:331-337 (2019). Read more (PubMed: 30822282) »
See all 8 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

IL-10 ELISA kit

Average Below average 2/5 (Ease of Use)
Abreviews
Standard curve is ok. Worked well but it was overexposed in 7 minutes and it's written 20 minutes in the manual. Found some extra stuff in the kit which was not written in "material supplied" table.

Miss. Sheelu Monga

Verified customer

投稿 Mar 27 2018

Question
Answer

Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test whole blood).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question
Answer

Merci de nous avoir contactés.

L'anticorps anti-IL10 du kit ab108870 est un polyclonal de lapin biotinylé dont l'immunogène est la séquence complète d'IL10 de souris.
La référence catalogue correspondant à cet anticorps est ab9735, le lien vers la fiche technique est www.abcam.com/ab9735. Veuillez noter que le ab9735 est non-conjugué.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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