製品の概要

  • 製品名

    Mouse IL-10 ELISA Kit
    IL-10 キット 製品一覧
  • 検出方法

    Colorimetric
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 45 pg/ml
  • 検出範囲

    78.13 pg/ml - 5000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 103.7 79% - 120%
    Serum 85.97 77% - 93%
    Plasma 92.2 71% - 134%

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    Abcam’s Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100697

  • Representative standard curve using ab100697

プロトコール

参考文献

This product has been referenced in:

  • Tyrtyshnaia A  et al. Neuroinflammation and adult hippocampal neurogenesis in neuropathic pain and alkyl glycerol ethers treatment in aged mice. Int J Mol Med 43:2153-2163 (2019). Read more (PubMed: 30896810) »
  • You P  et al. Local level of TGF-ß1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis. Exp Ther Med 15:3639-3649 (2018). Read more (PubMed: 29545894) »
See all 3 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Answer

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100566 IL2 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-2 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100570 IL4 Human ELISA Kit
o Capture Antibody
§ Mouse, monoclonal
§ Not tested for cross-reactivity with mouse samples.
o Detection Antibody
§ Goat, polyclonal
§ Detects the region His25­Ser153 (Accession # NP_000580)
§ Not tested for cross-reactivity with mouse samples.
· ab100689 Interferon gamma Mouse ELISA Kit
o Capture Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
· ab100697 IL10 Mouse ELISA Kit
o Capture Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
· ab100706 IL2 Mouse ELISA Kit
o The detection antibody did not show any cross-reactivity with Human IL-2 from testing. Thus, this kit is not expected to cross react with human samples.
· ab100710 IL4 Mouse ELISA Kit
o The capture antibody did not show any cross-reactivity with Human IL-4 from testing. Thus, this kit is not expected to cross react with human samples.

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Question
Answer

Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test whole blood).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Abreview for IL10 Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Tissue samples were homogenised in T-PER (Thermo) and protein concentration measured at 5mg/ml with a BCA Assay. These could be diluted 1:5 - 1:100 in the Assay Diluent and incubated overnight at 4oC and the results were measurable with the kit.

Abcam user community

Verified customer

投稿 Aug 17 2012

Answer

Thank you for your inquiry.

I can confirm that these kit are suitable for the use with serum samples that have been frozen.

I recommend to not to mix/compare samples thathave been frozen with samples that have not been frozen unless the impact of freezing is tested in a control experiment first.

I hope this information is helpful and wish you good luck with your experiments.

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