Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Mouse IgM, kappa monoclonal [MM-30] - isotype control (ab18401)

Q&A (2)References (6)

製品の概要

  • 製品名

    Mouse IgM, kappa monoclonal [MM-30] - isotype control
    Mouse アイソタイプ・コントロール 製品一覧

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, WB, IP, Flow Cytmore details

  • 免疫原

    KLH

  • 特記事項

    This immunoglobulin reacts with KLH.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 特記事項（精製）

    The immunoglobulin was purified by affinity chromatography.

  • アイソタイプ・コントロール 備考

    This immunoglobulin reacts with KLH.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MM-30

  • アイソタイプ

    IgM

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18401の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

参考文献 (6)

ab18401 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18401 は 6 報の論文で使用されています。

  • Tao L  et al. Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells. Nat Microbiol 4:1760-1769 (2019). PubMed: 31160825
  • Klaus T  et al. Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Sci Rep 8:519 (2018). PubMed: 29323348
  • Tian S  et al. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PLoS Biol 16:e2006951 (2018). PubMed: 30481169
  • Klaus T  et al. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep 6:30938 (2016). PubMed: 27484487
  • Karén J  et al. Effects of the histone deacetylase inhibitor valproic Acid on human pericytes in vitro. PLoS One 6:e24954 (2011). Flow Cyt . PubMed: 21966390
  • Sobol M  et al. UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity. Nucleus 4:478-86 (0). PubMed: 24513678

レビューと Q&A

Q&A
質問を送る

1-2 of 2 Q&A

Question

What are the differences between then? I saw that same are Kappa ans one is ICIGM?





































Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body


[CCE3749300]







Discover more at abcam.com

Read More
Answer

Thank you for your reply.

All 3 isotype controls are mouse monoclonal antibodies.

Two of them have a kappa light chain (ab18400 and ab18401). For ab91545, we do not have information about the type of light chain (either kappa or lambda).

ICIGM is the clone number. All clone numbers are given in brackets [...] in our catalog.

The antibodiesdiffer in the tested applications, concentration and buffer composition and amount you would receive (100 ug or 500 ug).

You might want to choose the one of these 3 controls that comes closest to your primary antibody in terms of concentration, but in general any of these isotype controls would be suitable for your IHC experiment.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

The CD3 antibody that I have here is not from ABCAM.

I am looking for a negative control IgM, to be used in immunhistochemistry slides.

Read More
Answer

Thank you for contacting us.

We carry several isotype controls that are mouse IgM:

ab91545: https://www.abcam.com/Mouse-IgM-ICIGM-Isotype-control-ab91545.html

ab18400: https://www.abcam.com/Mouse-IgM-kappa-MM-30-low-endotoxin-isotype-control-ab18400.html

ab18401: https://www.abcam.com/Mouse-IgM-kappa-monoclonal-MM-30-isotype-control-ab18401.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com