Mouse IgM, kappa monoclonal [MM-30] - isotype control (ab18401)

Q&A (2)References (4)

製品の概要

  • 製品名

    Mouse IgM, kappa monoclonal [MM-30] - isotype control
    Mouse アイソタイプ・コントロール 製品一覧

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, WB, IP, Flow Cytmore details

  • 免疫原

    KLH

  • 特記事項

    This immunoglobulin reacts with KLH.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 特記事項（精製）

    The immunoglobulin was purified by affinity chromatography.

  • アイソタイプ・コントロール 備考

    This immunoglobulin reacts with KLH.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MM-30

  • アイソタイプ

    IgM

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

Our Abpromise guarantee covers the use of ab18401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

参考文献 (4)

ab18401 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18401 は 4 報の論文で使用されています。

  • Klaus T  et al. Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Sci Rep 8:519 (2018). PubMed: 29323348
  • Tian S  et al. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PLoS Biol 16:e2006951 (2018). PubMed: 30481169
  • Klaus T  et al. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep 6:30938 (2016). PubMed: 27484487
  • Karén J  et al. Effects of the histone deacetylase inhibitor valproic Acid on human pericytes in vitro. PLoS One 6:e24954 (2011). Flow Cyt . PubMed: 21966390

レビューと Q&A

Q&A
質問を送る

1-2 of 2 Q&A

Question

What are the differences between then? I saw that same are Kappa ans one is ICIGM?





































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Answer

Thank you for your reply.

All 3 isotype controls are mouse monoclonal antibodies.

Two of them have a kappa light chain (ab18400 and ab18401). For ab91545, we do not have information about the type of light chain (either kappa or lambda).

ICIGM is the clone number. All clone numbers are given in brackets [...] in our catalog.

The antibodiesdiffer in the tested applications, concentration and buffer composition and amount you would receive (100 ug or 500 ug).

You might want to choose the one of these 3 controls that comes closest to your primary antibody in terms of concentration, but in general any of these isotype controls would be suitable for your IHC experiment.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

The CD3 antibody that I have here is not from ABCAM.

I am looking for a negative control IgM, to be used in immunhistochemistry slides.

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Answer

Thank you for contacting us.

We carry several isotype controls that are mouse IgM:

ab91545: https://www.abcam.com/Mouse-IgM-ICIGM-Isotype-control-ab91545.html

ab18400: https://www.abcam.com/Mouse-IgM-kappa-MM-30-low-endotoxin-isotype-control-ab18400.html

ab18401: https://www.abcam.com/Mouse-IgM-kappa-monoclonal-MM-30-isotype-control-ab18401.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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