Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab178002)
製品の概要
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製品名
Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control -
標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
特異性
ab178002 was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. ab178002 is a suitable control for Human, Rat, Mouse, Monkey, others untested. -
アプリケーション
適用あり: ICC/IFmore details -
特記事項
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
モノクローナル -
クローン名
7E10G10 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178002の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
画像
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Immunocytochemistry data for ab178002. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178002 at 1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 1 μg/mL (C).
Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C. Cells were blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3X with 1%GS and DAPI was added for 10 minutes in 1%GS.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab178002 は論文での使用が確認できていません。