Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)
Related conjugates and formulations
製品の概要
-
製品名
Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
Mouse アイソタイプ・コントロール 製品一覧 -
特異性
This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues. -
アプリケーション
適用あり: IP, WB, IHC-Fr, IHC-P, Flow Cyt, ChIP/Chip, ChIPmore details -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MOPC-173 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
-
別名
- Mouse Isotype Control
関連製品
-
Alternative Versions
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18413の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ChIP/Chip |
Use at an assay dependent concentration. PubMed: 18636108
|
|
ChIP |
Use at an assay dependent concentration. PubMed: 18636108
|
特記事項 |
---|
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ChIP/Chip
Use at an assay dependent concentration. PubMed: 18636108 |
ChIP
Use at an assay dependent concentration. PubMed: 18636108 |
画像
-
ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (55)
ab18413 は 55 報の論文で使用されています。
- Davari K et al. Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy. J Immunother Cancer 9:N/A (2021). PubMed: 33771892
- Li J et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994
- Li J et al. SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation. Nat Commun 12:784 (2021). PubMed: 33542242
- Li J et al. ELL Facilitates RNA Polymerase II-Mediated Transcription of Human Epidermal Proliferation Genes. J Invest Dermatol 141:1352-1356.e3 (2021). PubMed: 33157094
- Rinkunaite I et al. The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells. Biomolecules 11:N/A (2021). PubMed: 33921883