Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)

Q&A (3)References (25)

製品の概要

  • 製品名

    Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
    Mouse アイソタイプ・コントロール 製品一覧

  • 製品の詳細

    Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade

  • 特異性

    This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.

  • アプリケーション

    適用あり: Flow Cyt, ChIP/Chip, ChIPmore details

  • 種交差性

    交差種: Human

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ChIP/Chip Use at an assay dependent concentration. PubMed: 18636108
ChIP Use at an assay dependent concentration. PubMed: 18636108

画像

  • ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)
    ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.

参考文献 (25)

ab18413 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18413 は 25 報の論文で使用されています。

  • Bedenbender K  et al. Inflammation-mediated deacetylation of the ribonuclease 1 promoter via histone deacetylase 2 in endothelial cells. FASEB J 33:9017-9029 (2019). PubMed: 31039328
  • Hu T  et al. Neuroprotection induced by Navß2-knockdown in APP/PS1 transgenic neurons is associated with NEP regulation. Mol Med Rep 20:2002-2011 (2019). PubMed: 31257483
  • Ray RM  et al. Control of LDL Uptake in Human Cells by Targeting the LDLR Regulatory Long Non-coding RNA BM450697. Mol Ther Nucleic Acids 17:264-276 (2019). PubMed: 31279228
  • Sprague D  et al. Nonlinear sequence similarity between the Xist and Rsx long noncoding RNAs suggests shared functions of tandem repeat domains. RNA 25:1004-1019 (2019). PubMed: 31097619
  • Lian SL  et al. A SNP uncoupling Mina expression from the TGFß signaling pathway. Immun Inflamm Dis 6:58-71 (2018). PubMed: 28967702
View all Publications for this product

レビューと Q&A

Q&A
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1-3 of 3 Q&A

Question

What is the difference between ab 18413 and ab 18449?

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Answer

Thank you for your reply.

They are the same antibody (same clone), just that ab18449 has been screened for low endotoxin levels for use with cell culture.

I hope this information helps. Please contact us with any other questions.

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Question

What are differences between these two products that may affect a selection of an antibody control for ab 9324 for application in cultured human hepatocytes?

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Answer

Thank you for contacting us.

The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

Therefore, you should use the mouse control IgG2a ab18413 and NOT the rabbit IgG ab37415.

To learn more about isotype controls, please see the link below:

https://www.abcam.com/index.html?pageconfig=technicalfaqs#5

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Inquiry: It is indicated in the description of ab18413 that it is Mouse Control IgG, but when you scroll down to the isotype paragraph of this product, you will see that it is IgG2a. Please clarify if ab18413 is Mouse Control IgG or Mouse Control IgG2a. The customer works with 2 primary antibodies. One is IgG2a and the second is IgG1 and she is looking for one isotype control for IHC-P that will fit both primary antibodies, that's why it is important for her to be sure that ab18413 is Mouse Control IgG and not only Mouse Control IgG2a. Thank you very much for your assistance and reply. Have a nice day.

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Answer

Thank you for contacting us.

I can confirm that ab18413 is isotype IgG2a, therefore is only suitable for one of the primary antibodies the customer is using. I have asked our products team to update the datasheet.

Apologies for any confusion.

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