Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
製品の概要
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製品名
Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control
Mouse アイソタイプ・コントロール 製品一覧 -
アプリケーション
適用あり: Flow Cyt, IHC-P, IHC-Frmore details -
免疫原
Other Immunogen Type corresponding to Mouse. Synthetic hapten
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特記事項
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab91353 is purified and passed through a 0.22µm filter -
アイソタイプ・コントロール 備考
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections -
ポリ/モノ
モノクローナル -
クローン名
B11/6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
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別名
- Mouse Isotype Control
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 2µg for 106 cells.
ab91353 is a negative isotype control |
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use 2µg for 106 cells. ab91353 is a negative isotype control |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Karanovic et al One. 2016 Aug 25;11(8):e0161706. doi: 10.1371/journal.pone.0161706. eCollection 2016. Fig 9. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Glomerular nestin expression in experimental groups.
(A) Negative control (×200).
(C) SHADR group: Diffuse glomerular nestin expression was detected involving almost all podocytes within glomerulus. After losartan and tempol treatment, either single or in combination, kidneys restored nestin expression similar to controls (SHC group).
Immunostaining was applied on 5 μm thick paraffin sections. After deparaffinization and rehydration, the sections were treated by microwave for 20 minutes at 400 W in citrate buffer (pH 6.0). After antigen retrieval, samples were incubated for 1 hour at room temperature with primary antibody for nestin (dilution 1:100). Sections were then treated using 3-amino-9-ethylcarbazole (AEC) as substrate, and counterstained with hematoxylin. Negative controls were performed by omitting the first antibody and mouse monoclonal antibodies as isotype control mouse IgG1 (ab91353) antibody was also used.
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Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Hirakawa et al PLoS One. 2016 Aug 3;11(8):e0159912. doi: 10.1371/journal.pone.0159912. eCollection 2016. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Effects of hypoxia on mRNA expression of IGF1R and IGF1, and production of IGF1 in cancer cells.
Cell surface expression of IGF1R by FACScan analysis. IGF1R expression level of Panc-1, RWP-1, OCUP-AT, and MiaPaCa-2 cells was higher in hypoxia than that in normoxia.
Cells (2 x 106 cells/mL) were fixed with 2% paraformaldehyde and incubated in PBS with anti IGF1R antibody (ab16890, Abcam) or mouse IgG1- isotype control (ab91353, Abcam) for 30 minutes at 22°C. Cells were subsequently labeled with FITC-conjugated secondary antibody (1:500; ab96879, Abcam) for 30 minutes at 22°C.
The percentage of positive cells were calculated and compared with isotype-matched control-stained cells.
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Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab59509 (red line).
The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
プロトコール
参考文献 (28)
ab91353 は 28 報の論文で使用されています。
- Kobayashi M et al. Inhibition of autophagy in theca cells induces CYP17A1 and PAI-1 expression via ROS/p38 and JNK signalling during the development of polycystic ovary syndrome. Mol Cell Endocrinol 508:110792 (2020). PubMed: 32199904
- Li Y et al. Effect of integrin ß1 in the treatment of stress urinary incontinence by electrical stimulation. Mol Med Rep 19:4727-4734 (2019). PubMed: 31059065
- Rusu P et al. GPD1 Specifically Marks Dormant Glioma Stem Cells with a Distinct Metabolic Profile. Cell Stem Cell 25:241-257.e8 (2019). PubMed: 31303549
- Mills R et al. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF. J Immunol Res 2019:1845128 (2019). PubMed: 31098385
- Setthawong P et al. Generation of porcine induced-pluripotent stem cells from Sertoli cells. Theriogenology 127:32-40 (2019). PubMed: 30639694