Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Submit a question References (10)
Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Immunocytochemistry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

製品の概要

  • 製品名

    Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
    Mouse アイソタイプ・コントロール 製品一覧

  • 特異性

    This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.

  • アプリケーション

    適用あり: Flow Cyt, ICC, IHC-Pmore details

  • 免疫原

    Other Immunogen Type. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.

  • 特記事項

    This antibody clone is manufactured by Abcam.

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...

  • 精製度

    Ammonium Sulphate Precipitation

  • ポリ/モノ

    モノクローナル

  • クローン名

    15-6E10A7

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 1 µg/ml.
ICC
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration. PubMed: 26627833
特記事項
Flow Cyt
Use a concentration of 1 µg/ml.
ICC
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration. PubMed: 26627833

画像

  • Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

    An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

  • Immunocytochemistry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    Immunocytochemistry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).

    An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

  • ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Fluorescence ELISA.

    An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

参考文献 (10)

ab170190 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab170190 は 10 報の論文で使用されています。

  • Rao F  et al. Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration. Stem Cells Int 2019:2546367 (2019). PubMed: 31191669
  • Chen X  et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
  • Sun Q  et al. PEGylated TiO2 nanoparticles mediated inhibition of cell migration via integrin beta 1. Sci Technol Adv Mater 19:271-281 (2018). PubMed: 29707067
  • Dudka D  et al. Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments. Nat Commun 9:2042 (2018). PubMed: 29795284
  • Deng H  et al. Susceptibility of epithelial cells cultured from different regions of human cervix to HPV16-induced immortalization. PLoS One 13:e0199761 (2018). PubMed: 29944714
  • Xu JG  et al. TGF-ß1-induced differentiation of SHED into functional smooth muscle cells. Stem Cell Res Ther 8:10 (2017). PubMed: 28114966
  • Cui Y  et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt . PubMed: 26996236
  • Landa-Solís C  et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank N/A:N/A (2015). PubMed: 26220398
  • Ebisawa M  et al. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours. Vet J N/A:N/A (2015). IF . PubMed: 26364240
  • Douglas DN  et al. Oxidative stress attenuates lipid synthesis and increases mitochondrial fatty acid oxidation in hepatoma cells infected with hepatitis C virus. J Biol Chem N/A:N/A (2015). IHC-P . PubMed: 26627833

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