Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

製品の概要

  • 製品名

  • 特異性

    This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
  • アプリケーション

    適用あり: Flow Cyt, ICC, IHC-Pmore details
  • 免疫原

    Other Immunogen Type. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.

  • 特記事項

    This antibody clone is manufactured by Abcam.

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • 精製度

    Ammonium Sulphate Precipitation
  • ポリ/モノ

    モノクローナル
  • クローン名

    15-6E10A7
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 別名

    • CCDC113
    • Coiled-coil domain-containing protein 113 Gene names
    • DKFZp434N1418
    • HSPC065

アプリケーション

Our Abpromise guarantee covers the use of ab170190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 26627833

画像

  • Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

    An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

  • Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).

    An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

  • Fluorescence ELISA.

    An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

参考文献

This product has been referenced in:

  • Sun Q  et al. PEGylated TiO2 nanoparticles mediated inhibition of cell migration via integrin beta 1. Sci Technol Adv Mater 19:271-281 (2018). Read more (PubMed: 29707067) »
  • Dudka D  et al. Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments. Nat Commun 9:2042 (2018). Read more (PubMed: 29795284) »
See all 8 Publications for this product

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