Mouse I-TAC ELISA Kit (CXCL11) (ab100716)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 5.487 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
-
製品名
Mouse I-TAC ELISA Kit (CXCL11)
CXCL11 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
5.487 pg/ml - 4000 pg/ml -
添加回収試験
80 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 74.46 67% - 86% Serum 91.12 80% - 101% Plasma 75.6 68% - 88% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse I-TAC 2 vials I-TAC Microplate (12 x 8 wells) 1 unit Recombinant mouse I-TAC Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. -
組織特異性
High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- b R1
- b-R1
- Beta-R1
see all -
参照データベース
- Entrez Gene: 56066 Mouse
- SwissProt: Q9JHH5 Mouse
- Unigene: 131723 Mouse
画像
-
Typical standard curve
Representative standard curve using ab100716
-
Typical standard curveRepresentative standard curve using ab100716
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab100716 は 2 報の論文で使用されています。
- Tong G et al. MACC1 regulates PDL1 expression and tumor immunity through the c-Met/AKT/mTOR pathway in gastric cancer cells. Cancer Med 8:7044-7054 (2019). PubMed: 31557409
- Brenndörfer ED et al. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol 192:1671-80 (2014). Sandwich ELISA ; Mouse . PubMed: 24442435