Mouse Haptoglobin ELISA Kit (ab272472)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.91 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Haptoglobin ELISA Kit
Haptoglobin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 2.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 2.8% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3.91 pg/ml -
検出範囲
18.75 pg/ml - 1200 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 115 113% - 117% Urine 116 114% - 118% Serum 110 107% - 113% Hep Plasma 109 104% - 113% EDTA Plasma 106 103% - 108% Cit plasma 111 110% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse Haptoglobin ELISA kit (ab272472) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Haptoglobin protein in serum, urine, plasma and cell and tissue supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Haptoglobin with 3.91 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Haptoglobin, encoded by the HP gene and produced mostly by hepatic cells, is a major acute phase protein. Haptoglobin is known to bind to free hemoglobin released from erythrocytes with high affinity and inhibits its oxidative activity to allow hepatic recycling of the heme iron. Biosynthesis of haptoglobin can also occur in adipose and lung tissues to provide antioxidant and antimicrobial activity. Studies have shown haptoglobin to play a role in stimulation of angiogenesis and changes in the measured concentrations in serum may associate with certain inflammations, infections, and hemolytic conditions. Human, rat, cow, monkey share 81%, 90%, 76%, and 80% sequence homology with mouse haptoglobin, respectively.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse Haptoglobin Capture Antibody 1 x 600µl 10X Mouse Haptoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Haptoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Anhaptoglobinemia -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
参照データベース
- Entrez Gene: 15439 Mouse
- SwissProt: Q61646 Mouse
- Unigene: 26730 Mouse
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Haptoglobin standard curve in Sample Diluent NS.The Haptoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Haptoglobin in mouse serum and plasma samples.The concentrations of Haptoglobin were measured in duplicates, interpolated from the Haptoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:250,000, plasma (citrate) 1:1,000,000, plasma (EDTA) 1:250,000, and plasma (heparin) 1:250,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Haptoglobin concentration was determined to be 271.1 µg/mL in serum, 836.8 µg/mL in plasma (citrate), 265.2 µg/mL in plasma (EDTA), and 282.0 µg/mL in plasma (heparin).
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Interpolated concentrations of native Haptoglobin in mouse tissue culture supernatant and urine samples.The concentrations of Haptoglobin were measured in duplicates, interpolated from the Haptoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 1:200 and urine 1:160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Haptoglobin concentration was determined to be 211.7 ng/mL in liver supernatant and 109.3 ng/mL in urine.
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Sandwich ELISA - Mouse Haptoglobin ELISA Kit (ab272472)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272472 は論文での使用が確認できていません。