Mouse GRO gamma ELISA Kit (CXCL3) (ab272191)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.3 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse GRO gamma ELISA Kit (CXCL3)
GRO gamma キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 9.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 1.8% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.3 pg/ml -
検出範囲
6.25 pg/ml - 400 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 100 97% - 103% Serum 101 93% - 108% Hep Plasma 94 88% - 101% EDTA Plasma 94 86% - 93% Cit plasma 87 84% - 90% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse GRO gamma ELISA kit (ab272191) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GRO gamma protein in serum, plasmas and tissue culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GRO gamma with 1.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Chemokines are a group of small structurally related molecules that play fundamental roles in the development, homeostasis, and function of the immune system. GRO gamma (CXCL3) is an 8 kDa proinflammatory member of the chemokine heparin-binding CXC subfamily, or alpha chemokines, and is a ligand for CXCR2. GRO gamma has chemotactic activity for neutrophils and may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Mouse GRO gamma shares 62%, 90% and 63% sequence homology with human, rat and cow, respectively.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse GRO gamma (CXCL3) Capture Antibody 1 x 600µl 10X Mouse GRO gamma (CXCL3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse GRO gamma (CXCL3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-X-C motif chemokine 3
- C-X-C motif chemokine ligand 3
- Chemokine (C X C motif) ligand 3
see all -
参照データベース
- Entrez Gene: 330122 Mouse
- SwissProt: Q6W5C0 Mouse
関連製品
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Alternative Versions
画像
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Standard Curve comparison between the original Mouse GRO gamma SimpleStep ELISA kit (ab206310) and current Mouse GRO gamma SimpleStep ELISA kit (ab272191).
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The GRO gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of GRO gamma were measured in duplicates, interpolated from the GRO gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, plasma EDTA (100%) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GRO gamma concentration was determined to be 145.55 pg/mL in serum, 41.66 pg/mL in plasma (citrate), 31.17 pg/ml in plasma (EDTA) and 50.92 pg/mL in plasma (heparin).
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The concentrations of GRO gamma were measured in duplicate and interpolated from the GRO gamma standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GRO gamma concentration was determined to be 125 ng/mL in mouse lung supernatant treated with 5mg/ml LPS for 5 days and 18 ng/mL in mouse lung supernatants, mock treatment and 45.25 ng/ml in mouse spleen supernatant treated with 5mg/ml LPS for 5 days. *GRO gamma levels were assayed two times and found to be below the level of detection in mock treated mouse spleen supernatant.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272191 は論文での使用が確認できていません。