Mouse GPNMB ELISA Kit (ab270892)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 362 pg/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse GPNMB ELISA Kit
GPNMB キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Plasma 8 6.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Plasma 3 3.9% -
サンプルの種類
Serum, Cell culture media, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
362 pg/ml -
検出範囲
0.78 ng/ml - 50 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 108 101% - 116% Cell culture media 81 81% - 82% EDTA Plasma 110 104% - 120% Cit plasma 103 97% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse GPNMB ELISA kit (ab270892) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPNMB protein in mouse serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mouse GPNMB with 362 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
GPNMB (Glycoprotein non-metastatic gene B), also known as Osteoactivin, is a type I transmembrane glycoprotein most often associated with endosomal/lysosomal compartment and over expressed in many types of cancer. GPNMB is involved in many physiological processes, including transport of late melanosomes to keratinocytes, regulation of osteoblast and osteoclast, stimulation of dendritic cell maturation, and stimulation of adhesion of dendritic cells to endothelial cells.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse GPNMB Capture Antibody 1 x 600µl 10X Mouse GPNMB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse GPNMB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Could be a melanogenic enzyme. -
組織特異性
Not restricted to the melanocytic lineage. -
配列類似性
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
発生段階
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
細胞内局在
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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別名
- Glycoprotein (transmembrane) nmb
- Glycoprotein nmb
- Glycoprotein nmb like protein
see all -
参照データベース
- Entrez Gene: 93695 Mouse
- SwissProt: Q99P91 Mouse
- Unigene: 302602 Mouse
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse GPNMB standard curve in Sample Diluent 25BP.The GPNMB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GPNMB in mouse serum and plasma samples.The concentrations of GPNMB were measured in duplicates, interpolated from the GPNMB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GPNMB concentration was determined to be 19.4 ng/mL in neat serum, 6.5 ng/mL in plasma (citrate) and 6.6 ng/mL in plasma (EDTA).
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Sandwich ELISA - Mouse GPNMB ELISA Kit (ab270892)To learn more about the advantages of recombinant antibodies see here.
参考文献 (0)
ab270892 は論文での使用が確認できていません。
