Mouse Fibronectin ELISA Kit (ab108849)
Key features and details
- Sensitivity: 0.48 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Fibronectin ELISA Kit
Fibronectin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.6% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.48 ng/ml -
検出範囲
3.125 ng/ml - 200 ng/ml -
添加回収試験
101 %
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全工程の試験時間
3h 30m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse
交差が予測される動物種: Rat
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製品の概要
Mouse Fibronectin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108849) is designed for the quantitative measurement of Fibronectin levels in plasma, serum, urine and cell culture supernatants.
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse Fibronectin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Fibronectin Microplate (12 x 8 well strips) 1 unit Fibronectin Standard (lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
組織特異性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
関連疾患
Glomerulopathy with fibronectin deposits 2 -
配列類似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
発生段階
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻訳後修飾
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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別名
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
参照データベース
- Entrez Gene: 14268 Mouse
- Entrez Gene: 25661 Rat
- SwissProt: P11276 Mouse
- SwissProt: P04937 Rat
画像
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Sandwich ELISA - Ms fibronectin(ab108849)
Fibronectin measured in biological fluids showing quantity (ng) per mL of sample tested
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Typical standard CurveRepresentative Standard Curve using ab108849
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab108849 は 4 報の論文で使用されています。
- Kasagi Y et al. Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation. Cancer Res 76:347-57 (2016). PubMed: 26744523
- Jo DH et al. Quantitative Proteomics Reveals ß2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability. Mol Cell Proteomics 15:1681-91 (2016). PubMed: 26969716
- Brentnall M et al. Procaspase-3 regulates fibronectin secretion and influences adhesion, migration and survival independently of catalytic function. J Cell Sci 127:2217-26 (2014). PubMed: 24610949
- Riopel MM et al. ß1 integrin-extracellular matrix interactions are essential for maintaining exocrine pancreas architecture and function. Lab Invest N/A:N/A (2012). PubMed: 23069938