Mouse Fibrinogen ELISA Kit (ab108843)

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製品の概要

  • 製品名

    Mouse Fibrinogen ELISA Kit
    Fibrinogen キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 5.1%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 7.9%

  • サンプルの種類

    Cell culture supernatant, Urine

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    = 3 ng/ml

  • 検出範囲

    3.125 ng/ml - 200 ng/ml

  • 添加回収試験

    97.5 %

  • 全工程の試験時間

    4h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Mouse

  • 製品の概要

    Abcam’s Fibrinogen mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen concentrations in Mouse urine and cell culture media.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.


    Get better reproducibility in only 90 minutes with Mouse Fibrinogen ELISA Kit (ab213478) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50x Biotinylated Mouse Fibrinogen Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Fibrinogen Microplate (12 x 8 well strips) 1 unit
    Fibrinogen Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml

  • 研究分野

  • 機能

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • 組織特異性

    Plasma.

  • 関連疾患

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.

  • 配列類似性

    Contains 1 fibrinogen C-terminal domain.

  • ドメイン

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • 翻訳後修飾

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all

  • 参照データベース

    関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab108843 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent dilution.

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108843

    プロトコール

    参考文献

    This product has been referenced in:

    • Fields S  et al. New candidate biomarkers in the female genital tract to evaluate microbicide toxicity. PLoS One 9:e110980 (2014). Read more (PubMed: 25333937) »
    See 1 Publication for this product

    ab108843 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    What part of the fibrinogen protein does this antibody detect? I am looking for an antibody that is specific to the gamma chain of fibrinogen.

    Read More
    Answer

    This kit is not specific for the gamma chain; however, if you wanted to create your own ELISA assay we do have several antibodies that are specific to the gamma chain of fibrinogen.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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