Mouse CD30-L ELISA Kit (TNFSF8) (ab272476)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.6 pg/ml
- Range: 9.4 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
-
製品名
Mouse CD30-L ELISA Kit (TNFSF8)
CD30-L キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 3.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 4 9.7% -
サンプルの種類
Cell culture supernatant, Serum -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6.6 pg/ml -
検出範囲
9.4 pg/ml - 600 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 107 105% - 107% Serum 103 100% - 110% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse CD30-L ELISA kit (ab272467) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse CD30-L protein in serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD30-L with 6.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
特記事項
CD30-L, also known as CD153 and Tumor Necrosis Factor Ligand Superfamily Member 8 (TNFSF8), is a cytokine that is encoded by the gene TNFSF8. CD30-L is a single-pass type II membrane protein that forms a homotrimer. CD30-L is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma. Engagement of CD30-L expressed on B cell surfaces assists in modulating Ig class switching.
-
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse CD30-L (TNFSF8) Capture Antibody 1 x 600µl 10X Mouse CD30-L (TNFSF8) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse CD30-L (TNFSF8) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
-
機能
Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Membrane. - Information by UniProt
-
別名
- CD153
- CD153 antigen
- CD30 antigen ligand
see all -
参照データベース
- Entrez Gene: 21949 Mouse
- SwissProt: P32972 Mouse
- Unigene: 4664 Mouse
関連製品
-
Alternative Versions
画像
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of mouse CD30-L standard curve in Sample Diluent NS.The CD30-L standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native CD30-L in mouse EL4 stimulated and EL4 unstimulated.The concentrations of CD30-L were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: EL4 PHA stimulated 100% and EL4 unstimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 2,331 pg/mL in EL4 stimulated and 111 pg/mL in EL4 unstimulated.
-
Interpolated concentrations of native CD30-L in mouse serum.The concentrations of CD30-L were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 571 pg/mL in serum.
-
Sandwich ELISA - Mouse CD30-L ELISA Kit (TNFSF8) (ab272476)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272476 は論文での使用が確認できていません。