Mouse Antithrombin III ELISA Kit (SERPINC1) (ab108800)
Key features and details
- Sensitivity: 1.8 ng/ml
- Range: 6.25 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Antithrombin III ELISA Kit (SERPINC1)
Antithrombin III/ATIII キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 3.3% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 8.7% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 1.7 ng/ml -
検出範囲
6.5 ng/ml - 50 ng/ml -
添加回収試験
96 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.
An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse Antithrombin III Antibody 1 x 120µl Antithrombin III Standard 1 vial Antithrombin III Microplate (12 x 8 well strips) 1 unit Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
組織特異性
Found in plasma. -
関連疾患
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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別名
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
参照データベース
- Entrez Gene: 11905 Mouse
- SwissProt: P32261 Mouse
- Unigene: 260770 Mouse
画像
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Sandwich ELISA - Ms anti-thrombine III (ab108800)
Standard curve : mean of duplicates (+/-SD) with backgrounds readings subtracted
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Sandwich ELISA - Antithrombin III (SERPINC1) Mouse ELISA Kit (ab108800)Ms antithrombine III measured in biological fluids showing quantity (ng) per mL of sample tested
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108800 は 1 報の論文で使用されています。
- Foster DJ et al. Advanced siRNA Designs Further Improve In Vivo Performance of GalNAc-siRNA Conjugates. Mol Ther 26:708-717 (2018). PubMed: 29456020