アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    mouse-antithrombin-iii-elisa-kit-serpinc1-ab108800.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Fibrinolysis / Thrombolysis
Share by email

Mouse Antithrombin III ELISA Kit (SERPINC1) (ab108800)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (5)References (1)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Sandwich ELISA - Ms anti-thrombine III (ab108800)
  • Sandwich ELISA - Antithrombin III (SERPINC1) Mouse ELISA Kit (ab108800)

Key features and details

  • Sensitivity: 1.8 ng/ml
  • Range: 6.25 ng/ml - 50 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

こちらの製品もご検討ください

キット
Product image
Tissue Factor Activity Assay Kit (Human, Colorimetric) (ab108906)
ELISA
Product image
Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (ab137994)
ELISA
Product image
Mouse IgE ELISA Kit (ab157718)

関連製品

製品の概要

  • 製品名

    Mouse Antithrombin III ELISA Kit (SERPINC1)
    Antithrombin III/ATIII キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.7%
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    = 1.7 ng/ml
  • 検出範囲

    6.5 ng/ml - 50 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間

    4h 00m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.


    An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse Antithrombin III Antibody 1 x 120µl
    Antithrombin III Standard 1 vial
    Antithrombin III Microplate (12 x 8 well strips) 1 unit
    Chromogen Substrate 1 x 7ml
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • 研究分野

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Protease inhibitors ELISA kits
  • 機能

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 組織特異性

    Found in plasma.
  • 関連疾患

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • 配列類似性

    Belongs to the serpin family.
  • 翻訳後修飾

    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在

    Secreted > extracellular space.
  • Target information above from: UniProt accession P01008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 別名

    • ANT3_HUMAN
    • Antithrombin
    • Antithrombin III
    • Antithrombin-III
    • AntithrombinIII
    • AT 3
    • AT III
    • AT3
    • AT3D
    • ATIII
    • Heparin cofactor I
    • MGC22579
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1
    • Serine cysteine proteinase inhibitor clade C member 1
    • Serine proteinase inhibitor clade C member 1
    • Serpin C1
    • Serpin family C member 1
    • Serpin peptidase inhibitor clade C (antithrombin) member 1
    • SERPINC1
    • THPH7
    see all
  • 参照データベース

    • Entrez Gene: 11905 Mouse
    • SwissProt: P32261 Mouse
    • Unigene: 260770 Mouse

    画像

    • Sandwich ELISA - Ms anti-thrombine III (ab108800)
      Sandwich ELISA - Ms anti-thrombine III (ab108800)

      Standard curve : mean of duplicates (+/-SD) with backgrounds readings subtracted

    • Sandwich ELISA - Antithrombin III (SERPINC1) Mouse ELISA Kit (ab108800)
      Sandwich ELISA - Antithrombin III (SERPINC1) Mouse ELISA Kit (ab108800)

      Ms antithrombine III measured in biological fluids showing quantity (ng) per mL of sample tested

    プロトコール

    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (1)

    ab108800 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108800 は 1 報の論文で使用されています。

    • Foster DJ  et al. Advanced siRNA Designs Further Improve In Vivo Performance of GalNAc-siRNA Conjugates. Mol Ther 26:708-717 (2018). PubMed: 29456020

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question

    There is a great deal of variability between lots with the standards, which causes the same samples run with different lots to give different readings.

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    Thank you for your phone call.
    The standards for these kits are supplied in different masses. The mass for each vial will be printed on the label, and each lot will require a different volume of diluent in order to generate the 100 ng/mL standard. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on May 01 2012

    Question

    biotinylated mouse antibody, lot GR81436-1
    vial says 50x
    sent protocol says 80x
    online protocol says: 50x

    we switched from 50x (140 ul) to 80x (100 ul)
    ensure updates

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for your patience.

    Yes, I can confirm that the vial you received is 50x which is correct in this case - for the lot you received. We are looking into updating the datasheet. Going forward 80x will be the correct amount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Question

    biotinylated mouse antibody, lot GR81436-1
    vial says 50x
    sent protocol says 80x
    online protocol says: 50x

    we switched from 50x (140 ul) to 80x (100 ul)
    ensure updates

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2012

    Answer

    Thank you for contacting us.

    I have contacted the corresponding department regarding your inquiry and hope to have an answer for you soon. I will be in touch.

    I am sorryfor the confusion, and thank you for your patience.

    Read More

    Abcam Scientific Support

    Answered on Apr 16 2012

    Question

    I have been able to successfully plot the standard curve. See attached file. I tested serum and citrated plasma from naïve male CD1 mice, and obtained AT3 levels in the range of 400-500 ug/ml (after factoring in the 1:16,000 dilution). Are these values normal?  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 19 2011

    Answer

    Thank you for contacting us. I have had a chance to speak with the lab regarding your question. Our data suggested that mouse serum ATIII has slightly higher concentration than plasma. We have about 200 – 600 ug/ml range of different mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Abcam Scientific Support

    Answered on Dec 19 2011

    Question

    What are the expected baseline levels in serum?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Thank you for contacting us. I was hoping that you might fill me in on a couple of points about your results with this assay. Have you been able to plot the standard curve accurately using the mean value of the triplicate readings for each standard and sample?  The minimum detectable dose of mATIII is typically 1ng/ml. What readings are you seeing with your sample?   Thank you in advance for getting this information for me. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.