Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)
製品の概要
-
製品名
Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer
HRP/DAB detection kit キット 製品一覧 -
検出方法
Colorimetric -
アッセイタイプ
Micro-Polymer -
製品の概要
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit ab80436, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
-
特記事項
The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
-
アプリケーション
適用あり: IHC-Pmore details
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 15 ml 60 ml 125 ml 50x DAB Chromogen 1 x 0.5ml 1 x 2ml 1 x 4ml DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml Protein block 1 x 15ml 1 x 60ml 1 x 125ml -
研究分野
-
関連性
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236466の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
画像
-
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skinImage from Arash et al., Sci Rep; 10(1):7154. doi: 10.1038/s41598-020-64071-8.
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin. (A) The upper panel shows representative MMP-2 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX, and the lower panel shows bar graphs representing the expression of MMP-2 in the skin of CTRL and MFS mice at 12 weeks of age with or without DOX. (B) The upper panel shows representative MMP-9 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX treatment. The lower panel shows bar graphs representing the expression of MMP-9 in the skin of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
-
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9Image from Arash et al., Sci Rep; 10(1):7154. doi: 10.1038/s41598-020-64071-8.Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the aorta.Immunohistochemistry staining images and bar graphs representing the expression of (A) MMP-2 and (B) MMP-9 in the aortic roots of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)ab236466 Mouse and Rabbit Specific HRP-DAB Detection IHC Kit on Human Tonsil staining CD20 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436 (this kit is identical to ab80436, except that it uses the same HRP polymer secondary antibody used by Abcam's R&D team for antibody validation). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (16)
ab236466 は 16 報の論文で使用されています。
- Bishnu A et al. Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance. Cell Death Dis 12:161 (2021). PubMed: 33558461
- Pereira JNB et al. Small intestine remodeling in male Goto-Kakizaki rats. Physiol Rep 9:e14755 (2021). PubMed: 33580916
- Okkay U et al. Achillea millefolium alleviates testicular damage in paclitaxel-intoxicated rats via attenuation of testicular oxido-inflammatory stress and apoptotic responses. Andrologia 53:e14028 (2021). PubMed: 33650701
- Huang Y et al. A Novel, Personalized Drug-Screening System for Platinum-Resistant Ovarian Cancer Patients: A Preliminary Clinical Report. Cancer Manag Res 13:2849-2867 (2021). PubMed: 33833569
- Topchu I et al. Musashi 2 (MSI2) expression as an independent prognostic biomarker in non-small cell lung cancer (NSCLC). J Thorac Dis 13:1370-1379 (2021). PubMed: 33841930