Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467)
製品の概要
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製品名
Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer -
検出方法
Colorimetric -
アッセイタイプ
Micro-Polymer -
製品の概要
This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
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特記事項
Other IHC detection products
For alternative kits, see Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468, and HRP-DAB micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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アプリケーション
適用あり: IHC-Pmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 15 ml 125 ml AEC Single Solution 1 x 15ml 1 x 125ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 125ml Hydrogen Peroxide Block 1 x 15ml 1 x 125ml Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 125ml Protein block 1 x 15ml 1 x 125ml -
研究分野
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関連性
Biotin-Free Polyvalent AEC detection is a biotin-free immunoenzymatic antigen detection system. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
関連製品
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Immunohistochemistry reagents
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Substrate reagent
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab236467 は 5 報の論文で使用されています。
- Vukicevic S et al. Bone morphogenetic protein 1.3 inhibition decreases scar formation and supports cardiomyocyte survival after myocardial infarction. Nat Commun 13:81 (2022). PubMed: 35013172
- Huang H et al. Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression. Cell Rep 34:108522 (2021). PubMed: 33406417
- Pauk M et al. Iron overload in aging Bmp6-/- mice induces exocrine pancreatic injury and fibrosis due to acinar cell loss. Int J Mol Med 47:N/A (2021). PubMed: 33649802
- Vukicevic S et al. Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits. J Tissue Eng Regen Med 14:147-159 (2020). PubMed: 31671243
- Gesualdi L et al. The PI3K/AKT Pathway Is Activated by HGF in NT2D1 Non-Seminoma Cells and Has a Role in the Modulation of Their Malignant Behavior. Int J Mol Sci 21:N/A (2020). PubMed: 33212946