Mouse Amphiregulin ELISA Kit (ab100668)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.1 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Amphiregulin ELISA Kit
Amphiregulin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
1.1 pg/ml - 800 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 85.25 76% - 95% Serum 130.1 120% - 138% Plasma 123.4 112% - 130% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Amphiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Amphiregulin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Amphiregulin Microplate (12 x 8 wells) 1 unit Biotinylated anti-Mouse Amphiregulin 2 vials Recombinant Mouse Amphiregulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
The mature secreted form of amphiregulin is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR; these are EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas. -
細胞内局在
Cell Membrane -
別名
- A REG
- AR
- AREG
see all -
参照データベース
- Entrez Gene: 11839 Mouse
- SwissProt: P31955 Mouse
画像
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Typical Standard Curve
Representative Standard Curve using ab100668.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab100668 は 3 報の論文で使用されています。
- Hoyer FF et al. Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge. Immunity 51:899-914.e7 (2019). PubMed: 31732166
- Yang B et al. Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass. Genes Cells 23:136-145 (2018). PubMed: 29341420
- Boire A et al. Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis. Cell 168:1101-1113.e13 (2017). PubMed: 28283064