Mouse Alpha 1 Antitrypsin ELISA Kit (ab205088)
Key features and details
- Sensitivity: 1.492 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Alpha 1 Antitrypsin ELISA Kit
alpha 1 Antitrypsin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum < 10% -
サンプルの種類
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.492 ng/ml -
検出範囲
6.25 ng/ml - 200 ng/ml -
添加回収試験
> 85 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 6.25ng/ml - 200ng/ml -
全工程の試験時間
2h 10m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Alpha 1 Antitrypsin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Alpha 1 Antitrypsin protein in mouse biological samples.
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特記事項
In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP Conjugated Enzyme Antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Anti-Mouse A1AT ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Mouse A1AT Calibrator (lyophilized) 1 vial Stop Solution 1 x 12ml -
研究分野
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機能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
組織特異性
Ubiquitous. Expressed in leukocytes and plasma. -
関連疾患
Alpha-1-antitrypsin deficiency -
配列類似性
Belongs to the serpin family. -
ドメイン
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻訳後修飾
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
細胞内局在
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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別名
- A1A
- A1AT
- A1AT_HUMAN
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab205088 は 4 報の論文で使用されています。
- Stearns K et al. Knockdown of Alpha-1 Antitrypsin with antisense oligonucleotide does not exacerbate smoke induced lung injury. PLoS One 16:e0246040 (2021). PubMed: 33539438
- Deuse T et al. Hypoimmune induced pluripotent stem cell-derived cell therapeutics treat cardiovascular and pulmonary diseases in immunocompetent allogeneic mice. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34244428
- Joshi R et al. Sex-specific differences in emphysema using a murine antisense oligonucleotide model of a-1 antitrypsin deficiency. Am J Physiol Lung Cell Mol Physiol 316:L1165-L1173 (2019). PubMed: 31017014
- Yang J et al. Berberine ameliorates non-alcoholic steatohepatitis in ApoE-/- mice. Exp Ther Med 14:4134-4140 (2017). PubMed: 29075339