Mouse alpha 1 acid Glycoprotein ELISA Kit (ab264605)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 111.4 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse alpha 1 acid Glycoprotein ELISA Kit
Alpha 1 Acid Glycoprotein/AGP キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 3.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 3.4% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
111.4 pg/ml -
検出範囲
156.25 pg/ml - 10000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 103 98% - 112% Serum 104 99% - 108% Hep Plasma 108 100% - 116% EDTA Plasma 111 108% - 114% Cit plasma 103 94% - 112% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse alpha 1 acid Glycoprotein ELISA Kit (ab264605) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 1 acid Glycoprotein protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse alpha 1 acid Glycoprotein with 111.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Alpha 1 acid glycoprotein 1 (ORM1) is a plasma protein involved in acute inflammation. Alpha 1 acid glycoprotein is a transport protein for various cellular ligands and synthetic drugs. Alpha 1 acid glycoprotein is expressed by the liver and is induced by glucocorticoids, IL-1 and IL-6.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse alpha 1 acid Glycoprotein Capture Antibody 1 x 600µl 10X Mouse alpha 1 acid Glycoprotein Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse alpha 1 acid Glycoprotein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
組織特異性
Expressed by the liver and secreted in plasma. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- A1AG1_HUMAN
- AGP
- AGP 1
see all -
参照データベース
- Entrez Gene: 18405 Mouse
- SwissProt: Q60590 Mouse
- Unigene: 4777 Mouse
関連製品
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Alternative Versions
画像
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The alpha 1 acid Glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 2,000, plasma (citrate) 1: 8,000, plasma (heparin) 1: 4,000, and plasma (EDTA) 1: 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 16.84 µg/mL in serum, 47.40 µg/mL in plasma (citrate), 24.08 µg/ml in plasma (heparin), and 17.47 µg/ml in plasma (EDTA).
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The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 10.66 ng/mL in liver supernatant.
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The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: male mouse urine 1: 256. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 1.94 µg/mL in male mouse urine.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab264605 は論文での使用が確認できていません。