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Mouse Albumin ELISA Kit (ab108792)

Reviews (1)Q&A (2)References (53)
Typical Standard Curve for ab108792 Mouse Albumin ELISA kit

    Key features and details

    • Sensitivity: 0.44 ng/ml
    • Range: 3.125 ng/ml - 50 ng/ml
    • Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    製品の概要

    • 製品名

      Mouse Albumin ELISA Kit
      Albumin キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall 5.2%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall 9.8%

    • サンプルの種類

      Cell culture supernatant, Urine, Serum, Plasma, Cell Lysate

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      > 0.28 ng/ml

    • 検出範囲

      0.781 ng/ml - 200 ng/ml

    • 添加回収試験

      97 %

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Mouse

    • 製品の概要

      Mouse Albumin ELISA kit (ab108792) is a sandwich ELISA kit designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.


      An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 試験プラットフォーム

      Microplate

    製品の特性

    画像

    • Typical Standard Curve for ab108792 Mouse Albumin ELISA kit
      Typical Standard Curve for ab108792 Mouse Albumin ELISA kit

      Representative Standard Curve Using ab108792.

    参考文献 (53)

    ab108792 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108792 は 53 報の論文で使用されています。

    • Garg M  et al. Cardiolipin-mediated PPAR? S112 phosphorylation impairs IL-10 production and inflammation resolution during bacterial pneumonia. Cell Rep 34:108736 (2021). PubMed: 33567272
    • Liu X  et al. Knockout of NGAL aggravates tubulointerstitial injury in a mouse model of diabetic nephropathy by enhancing oxidative stress and fibrosis. Exp Ther Med 21:321 (2021). PubMed: 33732294
    • Yang WY  et al. Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes. Bioeng Transl Med 6:e10212 (2021). PubMed: 34027096
    • Zhao J  et al. Bruton's tyrosine kinase regulates macrophage-induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med 48:N/A (2021). PubMed: 34278465
    • Hamzaoui M  et al. Soluble Epoxide Hydrolase Inhibition Prevents Experimental Type 4 Cardiorenal Syndrome. Front Mol Biosci 7:604042 (2020). PubMed: 33777999
    View all Publications for this product

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    1-3 of 3 Abreviews or Q&A

    measurements on bronchoalveolar lavage fluid

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    A 4000x dilution of the BALF was used with this kit to measure the amount of serum albumin present in the BALF. Mice were treated intranasally with 50 microliters of either LPS (0,5ug/ul) or PBS as control. BALF was performed at different time points post LPS/PBS application.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Isabel Remory

    Verified customer

    投稿 Jul 28 2020

    Question

    I am interested in purchasing your ELISA kits – ab108792 and ab119557, to detect changes in mouse hippocampal tissue supernatants. I have not used these kits before and do not want to use half the plate to determine the optimum condition for tissue preparation and supernatant dilution for your detection range. I was wondering if you have any advice or any previous unpublished data on whether your kits are suitable for brain tissue supernatants and what dilution range you would recommend?

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    Answer


    ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

    ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCytomix kit, which uses the same antibodies, was used for brain tissues. Therefore, I would assume that this kit would work on your samples however, I cannot guarantee this.

    The publication is the following and may be of interest to you:

    Nogo-Receptors NgR1 and NgR2 Do Not Mediate Regulation of CD4 T Helper Responses and CNS Repair in Experimental Autoimmune Encephalomyelitis, Karin Steinbach et al, http://www.plosone.org 8 November 2011 | Volume 6 | Issue 11 | e26341

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    Question

    Our customer purchased ab108792 and opened it this morning.   There were something strange so asked me. The standard concentration starts from 400ng/ml but, standard vial label mentioned it is ( 1 X 360ng ).  How can our customer make this to 400 or 800ng/ml?   And wash buffer and diluent concentrate have precipitate. She tried to dissolve it but couldn't. This kit has some problem?   Please let her know how can she use this kit.     Best regards,

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    Answer

    Thank you for your enquiry. I am sorry to hear the customer has some concerns regarding this kit, I hope I can be of help. For the standard,  as described in the protocol this needs to be reconstituted with the appropriate amount of diluent to generate a stock solution of 400 ng/ml.   If the customer has 360 ng of standard in their vial, they can calculate how much diluent to reconstitute this in to obtain a concentration of 400 ng/ml: 400 ng/ml divided by 360 ng/ml = 1.1 To work out the dilution factor, divide 1 ml by 1.1 = 0.909ml of diluent Therefore, dissolve the 360 ng in 909 ul of diluent, and this will give a concentration of 400 mg/ml. This can be used as the stock solution. Although the standard curve shown with the protocol goes up to 800 ng/ml, I think the protocol does not mention to use this concentration, because the graph is starting to plateau at this point, and is out of the linear range of the assay.  therefore, data collected at this higher concentration would not be accurate. For the wash buffer and diluent, I can suggest to mix them thoroughly, use a vortex mixer if necessary. Leave them at room temperature for half an hour to warm up the vial and help the precipitate to dissolve. I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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