Anti-Monoamine Oxidase A/MAO-A 抗体 [EPR7101] - BSA and Azide free (ab240031)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free
Monoamine Oxidase A/MAO-A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: human hepatocellular carcinoma, mouse liver, and rat liver tissues. ICC/IF: HepG2 cells.
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特記事項
ab240031 is the carrier-free version of ab126751.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 5.10 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7101 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751)
- Alexa Fluor® 647 Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200752)
- HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
- APC Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab310819)
- PE Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab310897)
- Alexa Fluor® 488 Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab310971)
- Alexa Fluor® 594 Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab311690)
- Alexa Fluor® 568 Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab312966)
- Alexa Fluor® 555 Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab313175)
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab240031の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. -
組織特異性
Heart, liver, duodenum, blood vessels and kidney. -
関連疾患
Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. -
配列類似性
Belongs to the flavin monoamine oxidase family. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4128 Human
- Entrez Gene: 17161 Mouse
- Entrez Gene: 29253 Rat
- Omim: 309850 Human
- SwissProt: P21397 Human
- SwissProt: Q64133 Mouse
- SwissProt: P21396 Rat
- Unigene: 183109 Human
see all -
別名
- Amine oxidase [flavin containing] A antibody
- Amine oxidase [flavin-containing] A antibody
- AOFA antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:100 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751) -
All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MAOA (Monoamine Oxidase A) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 60 kDaLanes 1 - 2: Merged signal (red and green). Green - ab126751 observed at 60 kDa. Red - loading control, ab8245, observed at 38 kDa.
ab126751 was shown to recognize Monoamine Oxidase A in wild-type HAP1 cells as signal was lost at the expected MW in MAOA (Monoamine Oxidase A) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MAOA (Monoamine Oxidase A) knockout samples were subjected to SDS-PAGE. Ab126751 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).
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ab126751 (purified) staining Monoamine Oxidase A/MAO-A in the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).
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ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human colon tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human kidney tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126751).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab240031 は論文での使用が確認できていません。