Anti-Monoamine Oxidase A/MAO-A 抗体 [EPR7101] (ab126751)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101]
Monoamine Oxidase A/MAO-A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7101] to Monoamine Oxidase A/MAO-A -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Monoamine Oxidase A/MAO-A aa 450-550. The exact sequence is proprietary.
(Peptide available asab196045) -
ポジティブ・コントロール
- WB: HepG2, Rat brain, and Mouse brain lysates. IHC-P: human hepatocellular carcinoma, mouse liver, and rat liver tissues. ICC/IF: HepG2 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 5.10 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7101 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Alexa Fluor® 647 Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200752)
- HRP Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab200928)
- Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] - BSA and Azide free (ab240031)
- Alexa Fluor® 568 Anti-Monoamine Oxidase A / MAO-A antibody [EPR7101] (ab312966)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
IHC-P | (2) |
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
ICC/IF |
1/50 - 1/100.
|
特記事項 |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
-
機能
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. -
組織特異性
Heart, liver, duodenum, blood vessels and kidney. -
関連疾患
Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. -
配列類似性
Belongs to the flavin monoamine oxidase family. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 4128 Human
- Entrez Gene: 17161 Mouse
- Entrez Gene: 29253 Rat
- Omim: 309850 Human
- SwissProt: P21397 Human
- SwissProt: Q64133 Mouse
- SwissProt: P21396 Rat
- Unigene: 183109 Human
see all -
別名
- Amine oxidase [flavin containing] A antibody
- Amine oxidase [flavin-containing] A antibody
- AOFA antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:100 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Monoamine Oxidase A/MAO-A with Purified ab126751 at 1:400 dilution (0.38 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MAOA (Monoamine Oxidase A) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 60 kDaLanes 1 - 2: Merged signal (red and green). Green - ab126751 observed at 60 kDa. Red - loading control, ab8245, observed at 0 kDa.
ab126751 was shown to recognize Monoamine Oxidase A in wild-type HAP1 cells as signal was lost at the expected MW in MAOA (Monoamine Oxidase A) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MAOA (Monoamine Oxidase A) knockout samples were subjected to SDS-PAGE. Ab126751 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/10000 dilution (Purified) + Mouse brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/2000 dilution (unpurified) + Mouse brain cortex tissue lysate - whole at 20 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1 minute
-
ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab126751 (purified) staining Monoamine Oxidase A/MAO-A in the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
-
All lanes : Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (ab126751) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 lysate
Lane 2 : NCI-H460 lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
ab126751 (unpurified), at 1/50 dilution, staining Monoamine Oxidase A/MAO-A in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (39)
ab126751 は 39 報の論文で使用されています。
- Nyarko JNK et al. Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations. Methods Mol Biol 2558:123-141 (2023). PubMed: 36169860
- Wenzel TJ et al. An (Immuno) Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution Within the Cell. Methods Mol Biol 2558:143-161 (2023). PubMed: 36169861
- Santin Y et al. Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene Transfer. Methods Mol Biol 2558:163-170 (2023). PubMed: 36169862
- Meng XL et al. The clinical implications and molecular features of intrahepatic cholangiocarcinoma with perineural invasion. Hepatol Int 17:63-76 (2023). PubMed: 36418844
- Chinnasamy P et al. Increased adipose catecholamine levels and protection from obesity with loss of Allograft Inflammatory Factor-1. Nat Commun 14:38 (2023). PubMed: 36596796