Application
Western blot
Sample
Mouse Tissue lysate - whole (olfactory epithelium)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12.5%)
Loading amount
40 µg
Specification
olfactory epithelium
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%BSA
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 32 kDa
Positive control
The first line is molecular weight marker showing 32 and 37 kDa. The second line is OE tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 05 2011