Application
Western blot
Sample
African green monkey Cell lysate - whole cell (hepatocyte)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
hepatocyte
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Incubation time
45 minute(s) · Temperature: 24°C · Diluent: TBS 5% milk
Dilution
1/200
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 30 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 May 30 2008