製品の概要

  • 製品名
    Anti-mono methyl Arginine antibody [16B11]
    mono methyl Arginine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [16B11] to mono methyl Arginine
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with free and bound NG-monomethyl arginine. There is no cross reaction with either NG-NG-dimethyl arginine or arginine.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Species independent
  • 特記事項


    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/500.

ターゲット情報

  • 別名
    • Arginine (mono methyl) antibody

プロトコール

参考文献

This product has been referenced in:
  • de Groot R  et al. Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens. Mol Syst Biol 14:e8064 (2018). Read more (PubMed: 29363560) »
  • Poornima G  et al. Arginine methylation promotes translation repression activity of eIF4G-binding protein, Scd6. Nucleic Acids Res 44:9358-9368 (2016). Read more (PubMed: 27613419) »

See all 13 Publications for this product

ab414 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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4 reviews or Q&A

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BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 100537 DESCRIPTION OF THE PROBLEM I ordered three antibodies, ab412, ab413, and ab414. I am currently trying to optimize the conditions for Western blotting using these antibodies. None...

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Thank you for your enquiry. Thank you also for completing our questionnaire so comprehensively. I am sorry that you have been having difficulties with our antisera against methyl arginine. I have examined your questionnaire and it seems as if you a...

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I have recentrly tried to order a peptide with a monomethylated arginine, but this was not possible. How did you manage to prepare the antigen then for this monomethyl-arginine specific antibody?

Thank you for your enquiry. This antibody comes from a source which does not sell the monomethylated arginine immunogen used to prepare the antibody. I apologise for the inconvenience,

Concerning ab415, ab414, ab413 - says whole antiserum; is it tisue culture supernatant or ascites? Do ab412 and ab413 cross-reat with symmetrical Ng/Na - dimethyl arginine?

Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

What is tha difference between ab 414 and ab415?

There is no difference. They are simply different clones against the same target.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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