Anti-mono and dimethyl Arginine 抗体 [7E6] (ab412)

Abreviews (1)Q&A (39)Specific References (31)

製品の概要

  • 製品名
    Anti-mono and dimethyl Arginine antibody [7E6]
  • 製品の詳細
    Mouse monoclonal [7E6] to mono and dimethyl Arginine
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IP, ChIP, ELISAmore details
    適用なし: WB
  • 種交差性
    交差種: Species independent
  • 免疫原

    Asymmetrical NG/NG-dimethyl arginine

  • ポジティブ･コントロール
    • Methylated histones are an option
  • 特記事項

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises both dimethyl and monomethyl arginine it can be used in parallel with ab413 (detects dimethyl arginine only) and ab414 (detects monomethyl arginine only) to monitor the exact modification status. A tissue culture supernatant version is available as ab5394.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ChIP Use 3µl for 106 cells.
ELISA Use a concentration of 0.05 - 0.5 µg/ml.
  • 追加情報
    Is unsuitable for WB.

    • ターゲット情報

    • 別名
      • Di methyl Arginine antibody
      • Dimethyl Arginine antibody
      • Methyl Arginine antibody
      • Mono methyl Arginine antibody
      • Monomethyl Arginine antibody
      see all

    画像

    • ChIP - Anti-mono and dimethyl Arginine antibody [7E6] (ab412)
      ChIP - Anti-mono and dimethyl Arginine antibody [7E6] (ab412)This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK

      Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab412 to mono and dimethyl Arginine.

      Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab412 and 4x106 cells were used in each ChIP experiment.

    • Immunoprecipitation - Anti-mono and dimethyl Arginine antibody [7E6] (ab412)
      Immunoprecipitation - Anti-mono and dimethyl Arginine antibody [7E6] (ab412)Image from P Gupta et al, PLoS ONE 3:e2658 (2008), Fig 2.
      RIP140 null adipocytes extracts were suspended in 250 µl of immunoprecipitation buffer (150 mM NaCl,50 mM Tris-HCl (pH 8.0), 1 mM EDTA, 0.2% (v/v) Nonidet P40, 2 mM PMSF, 0.1% (w/v) SDS and a protease-inhibitor cocktail. Protein extracts (200 µg) were incubated with antibodies to RIP140, 14-3-3, or PRMT1 overnight at 4°C, and precipitated with protein G–agarose beads for 1–2 hours. ab412 was then used (amongst other antibodies) in Western Blot. Reduced protein inputs were used for ectopic expression of RIP140 to avoid saturation.

    参考文献

    This product has been referenced in:
    • Ganesan M  et al. Demethylase JMJD6 as a New Regulator of Interferon Signaling: Effects of HCV and Ethanol Metabolism. Cell Mol Gastroenterol Hepatol 5:101-112 (2018). Read more (PubMed: 29693039) »
    • Kong GM  et al. Selective small-chemical inhibitors of protein arginine methyltransferase 5 with anti-lung cancer activity. PLoS One 12:e0181601 (2017). Read more (PubMed: 28806746) »

    See all 31 Publications for this product

    ab412 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    40 reviews or Q&A

    10
    Votes: Highest First

    Immunoprecipitation abreview for Anti-mono and dimethyl Arginine [7E6] antibody

     Excellent
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (fibroblast)
    Specification
    fibroblast
    Total protein in input
    1000 µg
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jun 19 2014

    I need Discount code for the three below antibodies . Please arrange to send the same at your earliest .

    ab21623, ab23366 and ab412

    for detection of methylation and acetylation of bacterial proteins.

    Regards

    Thank you for contacting us.

    These antibodies are raised against targets that are not species specific. The antibodies would detect the proteins irrespective of species so the Abtrial discount will not be valid.

    I hope this informatio...

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    Dear Kate,

    thank you for your email. I would appreciate if you could send me a free of charge replacement.

    Thank you very much.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    1. Could you confirm if reducing and denaturing conditions have been tried? We recommend to use antibodies in reducing and denaturing conditions in WB unless it recommends to use non denaturing and reducing conditions on the datasheet. This will ensure...

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    Thank you for your message and for providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

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    Dear Madam/Sir,

    please see below. I don’t think the antibody ab412 works correctly.

    Kind regards,



    Order Details
    Antibody code: ab412

    Problem
    Choose: No signal

    Lot number...

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    Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would...

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    ab412 no signal in WB
    Using M2A mouse neuroblastoma cell line

    Thank you for your recent telephone call and for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and cover...

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    I noticed that Abcam has in its inventory anti mono- and di-methyl arginine antibody (ab412), and anti-methylated lysine (mono-methyl, dimethyl) antibody (ab23366).
    Could you please send us a small sample of the antibodies mentioned above for a te...

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    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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    Thank you.
    We prefer to get refund.
    Please advise how to proceed to receive the refund.

    Thank you for contacting us.
    Your credit note ID is *.
    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasin...

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    Please see our reponse. Thanks.
    1 - What sample type are you using? : we tried 293T cell lysates and mouse tissue (liver) lysates prepared in RIPA buffer.
    2 - Are you seeing any bands, no bands etc?: no clear bands, there are a few very faint ...

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    Thank you for your reply.
    I am sorry that the antibody is not working for you an based on the information that you have provided, I do not believe that there is ay protocol advice that I can offer that would resolve the issue.
    Therefore I would...

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    We have purchased below antibody (ab412 Mouse monoclonal [7E6] to mono and dimethyl Arginine)and tried it for WB application as recommended by the company. So far there is absolutely no signal we could detect under different conditions using various sa...

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    Thank you for contacting Abcam.
    The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on a variety of samples . If we cannot resolve the issue you are having with the antibody then I would be happy to ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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